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Flow cytometric analysis of MerTK (Mer) expression on viable Human U-937 cells. Cells from the U-937 (Histiocytic lymphoma, ATCC® CRL-1593™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human MerTK (Mer) antibody (Cat. No. 571778/571779, solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing MerTK (Mer) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.

Multicolor flow cytometric analysis of MerTK (Mer) expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein (50 ng/ml; PeproTech) for seven days. Dexamethasone (10 nM; Sigma-Aldrich) was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody (Cat. No. 550019) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human MerTK (Mer) antibody(Cat. No. 571778/571779, Right Plot). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK (Mer) [or Ig Isotype control staining] versus were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
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BD Pharmingen™ PE Mouse Anti-Human MerTK (Mer)

BD Pharmingen™ PE Mouse Anti-Human MerTK (Mer)
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The CC3-47 monoclonal antibody specifically recognizes Tyrosine-protein kinase Mer (MerTK) which is also simply known as MER due to its expression on monocytes, and in epithelial and reproductive tissue cells. MerTK (Mer) is a single-pass type I transmembrane glycoprotein that is encoded by MERTK (MER proto-oncogene, tyrosine kinase) which belongs to the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily of receptor tyrosine kinases (RTK). This kinase is comprised of an extracellular region with two immunoglobulin (Ig)-like domains followed by two fibronectin type III (FNIII) domains, a transmembrane segment, and a conserved intracellular tyrosine kinase domain. MerTK (Mer) is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells including retinal pigment epithelial cells. Through its extracellular Ig-like domains, MerTK (Mer) functions as a sensor for extracellular ligands such as the vitamin K-dependent Growth arrest-specific protein 6 (GAS6) and Protein S (PROS1). Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic MerTK (Mer) domains. This results in the activation of downstream signaling pathways that control cellular adhesion, aggregation, proliferation, survival, migration, and phagocytosis of apoptotic cells and cellular debris (efferocytosis). Through these activities, this kinase plays essential roles in the development and homeostasis of tissues including vascular, reproductive, and nervous systems. MerTK (Mer) is an essential regulator of hematopoiesis, inflammation, and innate and adaptive immunity. Abnormal expression and functioning of MerTK (Mer) is observed in various cancers and by certain tumor cell lines and linked to the development of various inflammatory disorders.

Development References (6)
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Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
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Graham DK, Dawson TL, Mullaney DL, Snodgrass HR, Earp HS. Cloning and mRNA expression analysis of a novel human protooncogene, c-mer.. Cell Growth Differ. 1994; 5(6):647-57. (Biology). View Reference
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Lemke G, Rothlin CV. Immunobiology of the TAM receptors. Nat Rev Immunol. 2008; 8(5):327-336. (Biology). View Reference
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Strick DJ, Vollrath D. Focus on molecules: MERTK.. Exp Eye Res. 2010; 91(6):786-7. (Biology). View Reference
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Zizzo G, Cohen PL. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.. J Immunol. 2013; 190(10):5237-46. (Biology). View Reference
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Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.. J Immunol. 2012; 189(7):3508-20. (Methodology). View Reference
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