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PE Mouse Anti-Human MerTK (Mer)
PE Mouse Anti-Human MerTK (Mer)
Flow cytometric analysis of MerTK (Mer) expression on viable Human U-937 cells. Cells from the U-937 (Histiocytic lymphoma, ATCC® CRL-1593™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human MerTK (Mer) antibody (Cat. No. 571778/571779, solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing MerTK (Mer) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
PE Mouse Anti-Human MerTK (Mer)
Multicolor flow cytometric analysis of MerTK (Mer) expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein (50 ng/ml; PeproTech) for seven days. Dexamethasone (10 nM; Sigma-Aldrich) was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody (Cat. No. 550019) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human MerTK (Mer) antibody(Cat. No. 571778/571779, Right Plot). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK (Mer) [or Ig Isotype control staining] versus  were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Flow cytometric analysis of MerTK (Mer) expression on viable Human U-937 cells. Cells from the U-937 (Histiocytic lymphoma, ATCC® CRL-1593™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and then stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dotted line histogram) or PE Mouse Anti-Human MerTK (Mer) antibody (Cat. No. 571778/571779, solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing MerTK (Mer) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Multicolor flow cytometric analysis of MerTK (Mer) expression on viable Human monocyte-derived macrophages. Normal Human monocytes were cultured in complete RPMI-1640 medium with heat-inactivated fetal bovine serum and purified recombinant Human M-CSF protein (50 ng/ml; PeproTech) for seven days. Dexamethasone (10 nM; Sigma-Aldrich) was added for the final 3 days to promote M2 macrophage polarization. The macrophages were harvested and stained with APC Mouse Anti-Human CD11b/Mac-1 antibody (Cat. No. 550019) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; Left Plot) or PE Mouse Anti-Human MerTK (Mer) antibody(Cat. No. 571778/571779, Right Plot). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The pseudocolor density plot showing the correlated expression of MerTK (Mer) [or Ig Isotype control staining] versus  were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) macrophages. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Pharmingen™
MER; MERTK; MerTK (Mer); c-mer; receptor tyrosine kinase MerTK
Human (QC Testing)
Mouse IgG1, κ
Recombinant Human MerTK ECD Protein
Flow cytometry (Routinely Tested)
5 µl/test
10461
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  8. For U.S. patents that may apply, see bd.com/patents.
571778 Rev. 1
Antibody Details
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CC3-47

The CC3-47 monoclonal antibody specifically recognizes Tyrosine-protein kinase Mer (MerTK) which is also simply known as MER due to its expression on monocytes, and in epithelial and reproductive tissue cells. MerTK (Mer) is a single-pass type I transmembrane glycoprotein that is encoded by MERTK (MER proto-oncogene, tyrosine kinase) which belongs to the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily of receptor tyrosine kinases (RTK). This kinase is comprised of an extracellular region with two immunoglobulin (Ig)-like domains followed by two fibronectin type III (FNIII) domains, a transmembrane segment, and a conserved intracellular tyrosine kinase domain. MerTK (Mer) is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, and epithelial cells including retinal pigment epithelial cells. Through its extracellular Ig-like domains, MerTK (Mer) functions as a sensor for extracellular ligands such as the vitamin K-dependent Growth arrest-specific protein 6 (GAS6) and Protein S (PROS1). Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic MerTK (Mer) domains. This results in the activation of downstream signaling pathways that control cellular adhesion, aggregation, proliferation, survival, migration, and phagocytosis of apoptotic cells and cellular debris (efferocytosis). Through these activities, this kinase plays essential roles in the development and homeostasis of tissues including vascular, reproductive, and nervous systems. MerTK (Mer) is an essential regulator of hematopoiesis, inflammation, and innate and adaptive immunity. Abnormal expression and functioning of MerTK (Mer) is observed in various cancers and by certain tumor cell lines and linked to the development of various inflammatory disorders.

571778 Rev. 1
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
571778 Rev.1
Citations & References
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View product citations for antibody "571778" on CiteAb

Development References (6)

  1. Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
  2. Graham DK, Dawson TL, Mullaney DL, Snodgrass HR, Earp HS. Cloning and mRNA expression analysis of a novel human protooncogene, c-mer.. Cell Growth Differ. 1994; 5(6):647-57. (Biology). View Reference
  3. Lemke G, Rothlin CV. Immunobiology of the TAM receptors. Nat Rev Immunol. 2008; 8(5):327-336. (Biology). View Reference
  4. Strick DJ, Vollrath D. Focus on molecules: MERTK.. Exp Eye Res. 2010; 91(6):786-7. (Biology). View Reference
  5. Zizzo G, Cohen PL. IL-17 stimulates differentiation of human anti-inflammatory macrophages and phagocytosis of apoptotic neutrophils in response to IL-10 and glucocorticoids.. J Immunol. 2013; 190(10):5237-46. (Biology). View Reference
  6. Zizzo G, Hilliard BA, Monestier M, Cohen PL. Efficient clearance of early apoptotic cells by human macrophages requires M2c polarization and MerTK induction.. J Immunol. 2012; 189(7):3508-20. (Methodology). View Reference
View All (6) View Less
571778 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.