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Flow cytometric analysis of LOX-1 (OLR1) expression on viable GM-CSF-stimulated Human peripheral blood monocytes. Adherent Human peripheral blood mononuclear cells (PBMC) were cultured for 3 days with Recombinant Human GM-CSF protein (Cat. No. 550068). The cultured cells were harvested and stained with either PE Mouse IgG2a, κ Isotype Control (Cat. No. 553457; dashed line histogram) or PE Mouse Anti-Human LOX-1 (OLR1) antibody (Cat. No. 571186/571187; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histogram showing LOX-1 (OLR1) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
BD Pharmingen™ PE Mouse Anti-Human LOX-1 (OLR1)
Regulatory Status Legend
Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.
Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The 15C4 monoclonal antibody specifically recognizes human Lectin-like oxidized LDL receptor 1 (LOX-1 or hLOX-1). LOX-1 is an ~31 kDa single pass type II membrane glycoprotein that contains an extracellular C-type lectin-like domain. LOX-1 is encoded by OLR1 (Oxidized low density lipoprotein receptor 1). This gene belongs to a subfamily of the NK cell C-type lectin-like (CLEC) receptor family and is likewise known as C-type lectin domain family 8 member A (CLEC8A). LOX-1 (OLR1) is expressed on macrophages, platelets, endothelial cells, fibroblasts, and smooth muscle cells. These cells can upregulate expression of the scavenger receptor in response to inflammatory and oxidative mediators. LOX-1 (OLR1) functions as a scavenger receptor identified as Scavenger receptor class E member 1 (SCARE1 or SR-E1) which plays a role in phagocytosis, endocytosis, and cytokine production. This receptor binds to multiple ligands including lipoproteins, oxidized LDL, C-reactive protein, and heat shock proteins released by apoptotic cells. It may play a role in vascular inflammation and atherosclerosis.
Development References (4)
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Duluc D, Gannevat J, Joo H, et al. Dendritic cells and vaccine design for sexually-transmitted diseases.. Microb Pathog. 2013; 58:35-44. (Clone-specific: Immunohistochemistry). View Reference
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Joo H, Li D, Dullaers M, et al. C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses.. Immunity. 2014; 41(4):592-604. (Clone-specific: Flow cytometry). View Reference
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Li D, Romain G, Flamar AL, et al. Targeting self- and foreign antigens to dendritic cells via DC-ASGPR generates IL-10-producing suppressive CD4+ T cells.. J Exp Med. 2012; 209(1):109-21. (Immunogen: Flow cytometry). View Reference
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Skinner JA, Zurawski SM, Sugimoto C, et al. Immunologic characterization of a rhesus macaque H1N1 challenge model for candidate influenza virus vaccine assessment.. Clin Vaccine Immunol. 2014; 21(12):1668-80. (Clone-specific: Functional assay). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.