BD Pharmingen™ PE Mouse Anti-Human CD301 (CLEC10A)
Clone MLD-1 (also known as MLD-1) (RUO)
Flow cytometric analysis of CD301 (CLEC10A) expression on Human peripheral blood CD1c+ dendritic cells. Human peripheral blood mononuclear cells (PBMC) were stained in CaCl2-supplemented HBSS, with Alexa Fluor™ 647 Mouse Anti-Human CD11c (Cat. No. 565912) and BD OptiBuild™ BV650 Mouse Anti-Human CD1c (Cat. No. 742749) antibodies and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CD301 (CLEC10A) antibody (Cat. No. 572154/572155; solid line histogram). The fluorescence histogram showing the expression of CD301 (CLEC10A) [or Ig Isotype control staining] was derived from CD1c+CD11c+ gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Multiparameter flow cytometric analysis of CD301 (CLEC10A) expression on Human peripheral blood leukocyte populations. Human peripheral blood cells were stained with Alexa Fluor™ 647 Mouse Anti-Human CD11c antibody (Cat. No. 565912) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human CD301 (CLEC10A) antibody (Cat. No. 572154/572155) as indicated. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Panel A – Human peripheral blood leukocyte populations. The bivariate pseudocolor density plot showing the correlated expression of CD301 (CLEC10A) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Panel B - Human peripheral blood monocytes. The bivariate pseudocolor density plot showing the correlated expression of CD301 (CLEC10A) [or Ig Isotype control staining] versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
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Flow cytometric analysis of CD301 (CLEC10A) expression on Human peripheral blood CD1c+ dendritic cells. Human peripheral blood mononuclear cells (PBMC) were stained in CaCl2-supplemented HBSS, with Alexa Fluor™ 647 Mouse Anti-Human CD11c (Cat. No. 565912) and BD OptiBuild™ BV650 Mouse Anti-Human CD1c (Cat. No. 742749) antibodies and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human CD301 (CLEC10A) antibody (Cat. No. 572154/572155; solid line histogram). The fluorescence histogram showing the expression of CD301 (CLEC10A) [or Ig Isotype control staining] was derived from CD1c+CD11c+ gated events with the forward and side light-scatter characteristics of viable dendritic cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Multiparameter flow cytometric analysis of CD301 (CLEC10A) expression on Human peripheral blood leukocyte populations. Human peripheral blood cells were stained with Alexa Fluor™ 647 Mouse Anti-Human CD11c antibody (Cat. No. 565912) and with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680) or PE Mouse Anti-Human CD301 (CLEC10A) antibody (Cat. No. 572154/572155) as indicated. Erythrocytes were lysed with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899). Panel A – Human peripheral blood leukocyte populations. The bivariate pseudocolor density plot showing the correlated expression of CD301 (CLEC10A) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of viable leukocyte populations. Panel B - Human peripheral blood monocytes. The bivariate pseudocolor density plot showing the correlated expression of CD301 (CLEC10A) [or Ig Isotype control staining] versus CD11c was derived from gated events with the forward and side light-scatter characteristics of viable monocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Product Details
BD Pharmingen™
C-type lectin domain family 10 member A; HML; HML2; MGL
Human (QC Testing)
Mouse BALB/c IgG1, κ
Recombinant ECD of MGL
Flow cytometry (Routinely Tested)
5 µl/test
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Clones without a listed workshop have not been investigated in an HLDA workshop to receive a CD nomenclature. We use “CD” provisionally when our internal testing indicates that this clone binds to the same CD antigen as workshopped clones.
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
PE Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
554680
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Alexa Fluor® 647 Mouse Anti-Human CD11c RUO
Size
0.1 mg
Cat No.
565912
572154 Rev. 1
Antibody Details
MLD-1
The MLD-1 monoclonal antibody specifically recognizes CD301 which is also known as C-type lectin domain family 10 member A (CLEC10A), Macrophage lectin 2, and Macrophage C-type lectin. CD301 (CLEC10A) is an ~40 kDa type II transmembrane glycoprotein that is encoded by CLEC10A which belongs to the C-type lectin superfamily. This lectin is comprised of an extracellular region with one carbohydrate recognition domain (CRD) and a neck region followed by a transmembrane segment and a cytoplasmic tail. CD301 (CLEC10A) is expressed on alternatively activated macrophages and immature dendritic cells and plays a role in the endocytosis and turnover of glycoproteins. It is involved in cellular adhesion and cell-cell signaling that mediate inflammatory and immune responses.
572154 Rev. 1
Format Details
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 561 nm
496 nm, 566 nm
576 nm
572154 Rev.1
Citations & References
View product citations for antibody "572154" on CiteAb
Development References (6)
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Heger L, Balk S, Lühr JJ, et al. CLEC10A Is a Specific Marker for Human CD1c+ Dendritic Cells and Enhances Their Toll-Like Receptor 7/8-Induced Cytokine Secretion.. Front Immunol. 2018; 9:744. (Biology). View Reference
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Heger L, Hofer TP, Bigley V, et al. Subsets of CD1c+ DCs: Dendritic Cell Versus Monocyte Lineage.. Front Immunol. 2020; 11:559166. (Biology). View Reference
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Heidkamp GF, Sander J, Lehmann CHK, et al. Human lymphoid organ dendritic cell identity is predominantly dictated by ontogeny, not tissue microenvironment.. Sci Immunol. 2016; 1(6):eaai7677. (Biology). View Reference
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Higashi N, Fujioka K, Denda-Nagai K, et al. The macrophage C-type lectin specific for galactose/N-acetylgalactosamine is an endocytic receptor expressed on monocyte-derived immature dendritic cells.. J Biol Chem. 2002; 277(23):20686-93. (Biology). View Reference
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Higashi N, Morikawa A, Fujioka K, et al. Human macrophage lectin specific for galactose/N-acetylgalactosamine is a marker for cells at an intermediate stage in their differentiation from monocytes into macrophages.. Int Immunol. 2002; 14(6):545-54. (Biology). View Reference
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Sano Y, Usami K, Izawa R, et al. Properties of blocking and non-blocking monoclonal antibodies specific for human macrophage galactose-type C-type lectin (MGL/ClecSF10A/CD301).. J Biochem. 2007; 141(1):127-36. (Immunogen: ELISA, Flow cytometry). View Reference
572154 Rev. 1
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.