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PE Mouse Anti-Human (CD255) TWEAK
Product Details
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BD Pharmingen™
CD255; TWEAK; TNFSF12; APO3 ligand; APO3L; DR3LG
Human (QC Testing)
Mouse BALB/c IgG3, κ
Human Tweak Transfected Cell Line
Flow cytometry (Routinely Tested)
20 µl
IX 96
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Please refer to for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at
  6. An isotype control should be used at the same concentration as the antibody of interest.
552831 Rev. 3
Antibody Details
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The CARL-1 monoclonal antibody specifically binds to CD255, also known as TWEAK (TNF-related WEAK inducer of apoptosis). The CD255 cytokine belongs to the tumor necrosis factor (TNF) ligand family and is encoded by the TNFSF12 (Tumor necrosis factor ligand superfamily member 12) gene. CD255 (TWEAK) is present as a type II transmembrane protein form and a smaller, biologically-active secreted isoform. CD255 is expressed on most tissues and non-lymphoid tumor cell lines but has weak or no expression on hematopoietic lineage cell lines. CD255 is not detectable on resting peripheral blood mononuclear cells. However, its expression is upregulated on peripheral blood monocytes in response to IFN-γ stimulation. CD255 can act through the TWEAK Receptor (aka, TWEAK-R, TNFRSF12A, Fn14, and CD266) to induce a variety of target cellular responses including NF-kB activation, proliferation, apoptosis, IL-8 and GM-CSF production. Clone CARL-2, a different monoclonal antibody, also binds to TWEAK although it binds to a different TWEAK epitope.

552831 Rev. 3
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
552831 Rev.3
Citations & References
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Development References (5)

  1. Chicheportiche Y, Bourdon PR, Xu H, et al. TWEAK, a new secreted ligand in the tumor necrosis factor family that weakly induces apoptosis. J Biol Chem. 1997; 272(51):32401-32410. (Biology). View Reference
  2. Kaplan MJ, Ray D, Mo RR, Yung RL, Richardson BC. TRAIL (Apo2 ligand) and TWEAK (Apo3 ligand) mediate CD4+ T cell killing of antigen-presenting macrophages. J Immunol. 2000; 164(6):2897-2904. (Biology). View Reference
  3. Llinas L, Lazaro A, de Salort J, Matesanz-Isabel J, Sintes J, Engel P. Expression profiles of novel cell surface molecules on B-cell subsets and plasma cells as analyzed by flow cytometry. Immunol Lett. 2011; 134(2):113-121. (Clone-specific: Flow cytometry). View Reference
  4. Lynch CN, Wang YC, Lund JK, Chen YW, Leal JA, Wiley SR. TWEAK induces angiogenesis and proliferation of endothelial cells. J Biol Chem. 1999; 274(13):8455-8459. (Biology). View Reference
  5. Nakayama M, Kayagaki N, Yamaguchi N, Okumura K, Yagita H. Involvement of TWEAK in interferon gamma-stimulated monocyte cytotoxicity. J Exp Med. 2000; 192(9):1373-1380. (Clone-specific: Blocking, Flow cytometry, Functional assay, Inhibition). View Reference
View All (5) View Less
552831 Rev. 3

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.