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      PE Mouse Anti-Human AXL
      PE Mouse Anti-Human AXL
      Multicolor flow cytometric analysis of AXL expression on Human A431 cells or Human peripheral blood mononuclear cells.    Left Plot: Cells from the Human A431 (Epidermoid Carcinoma, ATCC® CRL-1555™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human AXL antibody (Cat. No. 572242/572243; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing AXL expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Middle and Right Plots: Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block™. The cells were then stained with APC Mouse Anti-Human CD3 (Cat. No. 561811), APC Mouse Anti-Human CD19 (Cat. No. 555415), APC Mouse Anti-Human CD14 (Cat. No. 555399), APC Mouse Anti-Human CD15 (Cat. No. 551376), BD OptiBuild™ RB780 Mouse Anti-Human CD303 (BDCA-2) (Cat. No. 755529) and with either PE Mouse IgG1, κ Isotype Control (Middle Plot) or PE Mouse Anti-AXL antibody (Right Plot). DAPI Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of AXL (or Ig Isotype control staining) versus CD303 (BDCA-2) was derived from CD3-CD19-CD14-CD15- gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. The above is qualification data only and does not represent a specific OptiBuild™ lot.
      PE Mouse Anti-Human AXL
      Multicolor flow cytometric analysis of AXL expression on Human A431 cells or Human peripheral blood mononuclear cells.    Left Plot: Cells from the Human A431 (Epidermoid Carcinoma, ATCC® CRL-1555™) cell line were preincubated with Human BD Fc Block™ (Cat. No. 564220) and stained with either PE Mouse IgG1, κ Isotype Control (Cat. No. 554680; dashed line histogram) or PE Mouse Anti-Human AXL antibody (Cat. No. 572242/572243; solid line histogram). DAPI Solution (Cat. No. 564907) was added to cells right before analysis. The fluorescence histograms showing AXL expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Middle and Right Plots: Freshly isolated Human peripheral blood mononuclear cells were preincubated with Human BD Fc Block™. The cells were then stained with APC Mouse Anti-Human CD3 (Cat. No. 561811), APC Mouse Anti-Human CD19 (Cat. No. 555415), APC Mouse Anti-Human CD14 (Cat. No. 555399), APC Mouse Anti-Human CD15 (Cat. No. 551376), BD OptiBuild™ RB780 Mouse Anti-Human CD303 (BDCA-2) (Cat. No. 755529) and with either PE Mouse IgG1, κ Isotype Control (Middle Plot) or PE Mouse Anti-AXL antibody (Right Plot). DAPI Solution was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of AXL (or Ig Isotype control staining) versus CD303 (BDCA-2) was derived from CD3-CD19-CD14-CD15- gated events with the forward and side light-scatter characteristics of viable (DAPI-negative) cells.    Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software. The above is qualification data only and does not represent a specific OptiBuild™ lot.
      Product Details
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      BD Pharmingen™
      ARK; AXL; UFO; tyrosine-protein kinase receptor UFO
      Human (QC Testing)
      Mouse Swiss Webster IgG1, κ
      Recombinant Human AXL
      Flow cytometry (Routinely Tested)
      5 µl/test
      558
      Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

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      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      4. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. An isotype control should be used at the same concentration as the antibody of interest.
      7. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      8. For U.S. patents that may apply, see bd.com/patents.
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      572243 Rev. 1
      Antibody Details
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      DD6-362

      The DD6-362 monoclonal antibody specifically recognizes AXL which is also known as Adhesion related kinase (ARK), JTK11, Tyro7, or Tyrosine-protein kinase receptor UFO (UFO). Tyro-3, AXL, and Mer (MerTK) constitute the TAM subfamily of receptor tyrosine kinases (RTK). AXL is a single-pass type I transmembrane glycoprotein comprised of an extracellular region with two immunoglobulin (Ig)-like domains and two fibronectin type III (FNIII) domains, a transmembrane segment and a conserved intracellular tyrosine kinase domain. AXL is variably expressed by multiple cell types including monocytes, macrophages, dendritic cells, NK cells, platelets, endothelial cells, vascular smooth muscle cells, and fibroblasts. AXL binds to the vitamin K-dependent Growth arrest-specific protein 6 (Gas6) through its extracellular Ig-like domains. Ligand binding leads to receptor dimerization, and autophosphorylation of tyrosine residues within the cytoplasmic AXL domains. This results in the activation of downstream signaling pathways that control cellular adhesion, aggregation, phagocytosis/efferocytosis, proliferation, survival, and migration. Through these activities, AXL plays major roles in development, the regulation of hematopoiesis and immunity, and ensures the integrity of the vascular system. Abnormal expression of AXL has been observed in various cancers and myeloproliferative disorders and by tumor cell lines.

      572243 Rev. 1
      Format Details
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      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 561 nm
      496 nm, 566 nm
      576 nm
      572243 Rev.1
      Citations & References
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      View product citations for antibody "572243" on CiteAb

      Development References (9)

      1. Gould WR, Baxi SM, Schroeder R, et al. Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses.. J Thromb Haemost. 2005; 3(4):733-41. (Biology). View Reference
      2. Janssen JW, Schulz AS, Steenvoorden AC, et al. A novel putative tyrosine kinase receptor with oncogenic potential.. Oncogene. 1991; 6(11):2113-20. (Biology). View Reference
      3. Lemke G, Rothlin CV. Immunobiology of the TAM receptors. Nat Rev Immunol. 2008; 8(5):327-336. (Biology). View Reference
      4. Linger RM, Keating AK, Earp HS, Graham DK. TAM receptor tyrosine kinases: biologic functions, signaling, and potential therapeutic targeting in human cancer.. Adv Cancer Res. 2008; 100:35-83. (Biology). View Reference
      5. Prouse T, Majumder S, Majumder R. Functions of TAM Receptors and Ligands Protein S and Gas6 in Atherosclerosis and Cardiovascular Disease.. Int J Mol Sci. 2024; 25(23):12736. (Biology). View Reference
      6. Rothlin CV, Carrera-Silva EA, Bosurgi L, Ghosh S. TAM receptor signaling in immune homeostasis.. Annu Rev Immunol. 2015; 33:355-91. (Biology). View Reference
      7. Shiozawa Y, Pedersen EA, Patel LR, et al. GAS6/AXL axis regulates prostate cancer invasion, proliferation, and survival in the bone marrow niche.. Neoplasia. 2010; 12(2):116-27. (Biology: Flow cytometry). View Reference
      8. Taichman RS, Patel LR, Bedenis R, et al. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.. PLoS ONE. 2013; 8(4):e61873. (Biology: Flow cytometry). View Reference
      9. Yumoto K, Eber MR, Wang J, et al. Axl is required for TGF-β2-induced dormancy of prostate cancer cells in the bone marrow.. Sci Rep. 2016; 6:36520. (Biology). View Reference
      View All (9) View Less
      572243 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.