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PE-CF594 Mouse Anti-Human CD317 (BST2)
PE-CF594 Mouse Anti-Human CD317 (BST2)
Two-parameter flow cytometric analysis of CD317 (BST2) expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; Left Plot) or BD Horizon™ PE-CF594 Mouse Anti-Human CD317 (BST2) antibody (Cat. No. 568103/568104; Right Plot). Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of CD317 (BST2) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
PE-CF594 Mouse Anti-Human CD317 (BST2)
Two-parameter flow cytometric analysis of CD317 (BST2) expression on Rhesus peripheral blood leucocyte populations. Rhesus whole blood was stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; Left Plot) or BD Horizon™ PE-CF594 Mouse Anti-Human CD317 (BST2) antibody (Cat. No. 568103/568104; Right Plot). Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of CD317 (BST2) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Two-parameter flow cytometric analysis of CD317 (BST2) expression on Human peripheral blood leucocyte populations. Human whole blood was stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; Left Plot) or BD Horizon™ PE-CF594 Mouse Anti-Human CD317 (BST2) antibody (Cat. No. 568103/568104; Right Plot). Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of CD317 (BST2) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Two-parameter flow cytometric analysis of CD317 (BST2) expression on Rhesus peripheral blood leucocyte populations. Rhesus whole blood was stained with either BD Horizon™ PE-CF594 Mouse IgG2a, κ Isotype Control (Cat. No. 562306; Left Plot) or BD Horizon™ PE-CF594 Mouse Anti-Human CD317 (BST2) antibody (Cat. No. 568103/568104; Right Plot). Erythrocytes were lysed with BD FACS Lysing™ Solution (Cat. No. 349202). The pseudocolor density plot showing the correlated expression of CD317 (BST2) [or Ig Isotype control staining] versus side light-scatter (SSC-A) signals was derived from gated events with the forward and side light-scatter characteristics of intact leucocyte populations. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software.
Product Details
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BD Horizon™
BST2; BST-2; bone marrow stromal antigen 2; HM1.24 antigen; Tetherin
Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
Mouse BALB/c IgG2a, κ
Human KPC-32 Plasma Cell Line
Flow cytometry (Routinely Tested)
5 µl
VIII 80114
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Please observe the following precautions: Absorption of visible light can significantly alter the energy transfer occurring in any tandem fluorochrome conjugate; therefore, we recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to prevent exposure of conjugated reagents, including cells stained with those reagents, to room illumination.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  6. An isotype control should be used at the same concentration as the antibody of interest.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
  9. Texas Red is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  10. CF™ is a trademark of Biotium, Inc.
  11. When excited by the yellow-green (561-nm) laser, the fluorescence may be brighter than when excited by the blue (488-nm) laser.
  12. This product is provided under an Agreement between BIOTIUM and BD Biosciences. The manufacture, use, sale, offer for sale, or import of this product is subject to one or more patents or pending applications owned or licensed by Biotium, Inc. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
  13. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using multi-laser cytometers, which may directly excite both PE and CF™594.
  14. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Antibody Details
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Y129

The Y129 monoclonal antibody specifically binds to CD317 which is also known as Bone marrow stromal antigen 2 (BST2). CD317 is a type II transmembrane glycoprotein that belongs to the Tetherin family. CD317 is an interferon (IFN)-induced protein that is expressed on monocytes, granulocytes, dendritic cells, B lymphoblasts, plasma cells, T cells, natural killer (NK) cells, stromal cells and myeloma cells. CD317 is expressed by nonhematopoietic cells including cells within solid tumors derived from breast, lung and kidney. CD317 may be involved in the interactions between bone marrow stromal cells and lymphocytes. It is likewise known as Tetherin and reportedly blocks the release of some enveloped viruses by tethering virions to infected cell membranes. CD317 stimulates signaling by CD85g (ILT7) and may provide negative feedback for interferon (IFN) production by plasmacytoid dendritic cells during viral infections.

Format Details
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PE-CF594
BD Horizon™ PE-CF594 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye with an emission maximum (Em Max) at 615-nm. PE-CF594, driven by BD innovation, is designed to be excited by the blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 615 nm (e.g., a 610/20-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the green (532-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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PE-CF594
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
615 nm
Citations & References
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View product citations for antibody "568104" on CiteAb

Development References (11)

  1. Buckley CD, Halder S, Hardie D, et al. Report on antibodies submitted to the stromal cell section of HLDA8.. Cell Immunol. 236(1-2):29-41. (Immunogen). View Reference
  2. Gong S, Osei ES, Kaplan D, Chen YH, Meyerson H. CD317 is over-expressed in B-cell chronic lymphocytic leukemia, but not B-cell acute lymphoblastic leukemia.. Int J Clin Exp Pathol. 2015; 8(2):1613-21. (Biology). View Reference
  3. Goto T, Kennel SJ, Abe M, et al. A novel membrane antigen selectively expressed on terminally differentiated human B cells.. Blood. 1994; 84(6):1922-30. (Immunogen). View Reference
  4. Harada T, Ozaki S. Targeted therapy for HM1.24 (CD317) on multiple myeloma cells.. Biomed Res Int. 2014; 2014:965384. (Clone-specific: Functional assay, In vivo exacerbation, Radioimmunoassay). View Reference
  5. Mahauad-Fernandez WD, Okeoma CM. The role of BST-2/Tetherin in host protection and disease manifestation.. Immun Inflamm Dis. 2016; 4(1):4-23. (Biology). View Reference
  6. Neil SJ, Zang T, Bieniasz PD. Tetherin inhibits retrovirus release and is antagonized by HIV-1 Vpu.. Nature. 2008; 451(7177):425-30. (Biology). View Reference
  7. Serra-Moreno R, Jia B, Breed M, Alvarez X, Evans DT. Compensatory changes in the cytoplasmic tail of gp41 confer resistance to tetherin/BST-2 in a pathogenic nef-deleted SIV.. Cell Host Microbe. 2011; 9(1):46-57. (Clone-specific: Immunofluorescence). View Reference
  8. Staudinger M, Glorius P, Burger R, et al. The novel immunotoxin HM1.24-ETA' induces apoptosis in multiple myeloma cells.. Blood Cancer J. 2014; 4:e219. (Biology). View Reference
  9. Van Damme N, Goff D, Katsura C, et al. The interferon-induced protein BST-2 restricts HIV-1 release and is downregulated from the cell surface by the viral Vpu protein.. Cell Host Microbe. 2008; 3(4):245-52. (Clone-specific: Functional assay). View Reference
  10. Vidal-Laliena M, Romero X, March S, Requena V, Petriz J, Engel P. Characterization of antibodies submitted to the B cell section of the 8th Human Leukocyte Differentiation Antigens Workshop by flow cytometry and immunohistochemistry. Cell Immunol. 2005; 236(1-2):6-16. (Clone-specific: Calcium Flux, Flow cytometry). View Reference
  11. Wiche Salinas TR, Gosselin A, Raymond Marchand L, et al. IL-17A reprograms intestinal epithelial cells to facilitate HIV-1 replication and outgrowth in CD4+ T cells.. iScience. 2021; 24(11):103225. (Clone-specific: Flow cytometry). View Reference
View All (11) View Less

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.