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BV786 Mouse Anti-Human IgG
Two-color flow cytometric analysis of IgG expression on human peripheral blood lymphocytes. Human peripheral blood mononuclear cells were washed and cultured in complete tissue culture medium overnight in order to minimize subsequent nonspecific immunofluorescent staining. The cells were harvested and stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415/561742) and either BD Horizon™ BV786 Mouse IgG1, κ Isotype Control (Cat. No. 563330; Left Panel) or BD Horizon BV786 Mouse Anti-Human IgG antibody (Cat. No. 564230; Right Panel). Two-color contour plots showing the correlated expression of cell surface IgG (or Ig Isotype control staining) versus CD19 were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.
BD Horizon™ BV786 Mouse Anti-Human IgG
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