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BV786 Mouse Anti-Human CD337 (NKp30)
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This product is the replacement for 743172.
BV786 Mouse Anti-Human CD337 (NKp30)
Two-color flow cytometric analysis of CD337 (NKp30) expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 antibody (Cat. No. 561903/555516) and with either BD Horizon™ BV786 Mouse IgG1, κ Isotype Control (Cat. No. 563330; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD337 (NKp30) antibody (Cat. No. 568223; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD337 (NKp30) [or Ig Isotype control staining] versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Two-color flow cytometric analysis of CD337 (NKp30) expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Mouse Anti-Human CD56 antibody (Cat. No. 561903/555516) and with either BD Horizon™ BV786 Mouse IgG1, κ Isotype Control (Cat. No. 563330; Left Plot) or BD Horizon™ BV786 Mouse Anti-Human CD337 (NKp30) antibody (Cat. No. 568223; Right Plot) at 0.5 µg/test. Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plot showing the correlated expression of CD337 (NKp30) [or Ig Isotype control staining] versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Horizon™
NCR3; Natural cytotoxicity triggering receptor 3; NKp30; MALS; LY117; 1C7
Human (QC Testing)
Mouse IgG1, κ
Horse NKp30 extracellular domain
Flow cytometry (Routinely Tested)
0.2 mg/ml
259197
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation).  When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime BD Horizon Brilliant dyes are used in a multicolor flow cytometry panel.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. BD Horizon Brilliant Violet 786 is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,227,187; 8,455,613; 8,575,303; 8,354,239.
  5. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  7. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva.
  8. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  9. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  10. An isotype control should be used at the same concentration as the antibody of interest.
568223 Rev. 1
Antibody Details
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p30-15

The p30-15 monoclonal antibody specifically binds to CD337, also known as NKp30, a receptor found on the surface of natural killer (NK) cells.  NK cells are large lymphoid cells discovered because of their ability to recognize and kill abnormal cells such as tumor and virally infected cells.  NK cell immune responses are regulated by a balance of activating and inhibitory signals generated by cell surface receptors.  Inhibitory receptors recognize MHC class I molecules on normal cells producing a negative signal to the NK cell.  Loss of MHC class I expression in infected or transformed cells results in the loss of this negative signal leading to NK cell activation.  In concert with the loss of inhibitory signals, activation signals via NK receptors such as NKp30, NKp44, NKp46, NKG2D, and NKp80 mediate the activation of NK cells.  NKp30 cooperates with NKp46 and/or NKp44 in the induction of NK cell-mediated cytotoxicity against the majority of target cells.

568223 Rev. 1
Format Details
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BV786
The BD Horizon Brilliant Violet™ 786 (BV786) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an Ex Max of 407-nm and an acceptor dye with an Em Max at 786-nm.  BV786, driven by BD innovation, is designed to be excited by the violet laser and detected using a filter, centered near 785 nm (e.g. 780/60 nm bandpass filter).  Please ensure that your instrument’s configurations (lasers and filters) are appropriate for this dye.
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BV786
Violet 405 nm
407 nm
786 nm
568223 Rev.1
Citations & References
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Development References (5)

  1. Augugliaro R, Parolini S, Castriconi , et al. Selective cross-talk among natural cytotoxicity receptors in human natural killer cells. Eur J Immunol. 2003; 33(5):1235-1241. (Biology). View Reference
  2. Byrd A, Hoffmann SC, Jarahian M, Momburg F, Watzl C. Expression analysis of the ligands for the Natural Killer cell receptors NKp30 and NKp44. PLoS ONE. 2007; 2(12):e1339. (Immunogen: Blocking, ELISA, Flow cytometry). View Reference
  3. Flaig RM, Stark S, Watzl C. Cutting edge: NTB-A activates NK cells via homophilic interaction. J Immunol. 2004; 172(11):6524-6527. (Biology). View Reference
  4. Pende D, Parolini S, Pessino A, et al. Identification and molecular characterization of NKp30, a novel triggering receptor involved in natural cytotoxicity mediated by human natural killer cells. J Exp Med. 1999; 190(10):1505-1516. (Biology). View Reference
  5. Stark S, Flaig RM, Sandusky M, Watzl C. The use of trimeric isoleucine-zipper fusion proteins to study surface-receptor-ligand interactions in natural killer cells. J Immunol Methods. 2004; 296(1-2):149-158. (Biology). View Reference
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568223 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.