The 657909 monoclonal antibody specifically recognizes Tetraspanin-8 (TSPAN8) that belongs to the Transmembrane 4 (Tetraspanin) superfamily (TM4SF). This molecule is also known as Transmembrane 4 superfamily member 3 (TM4SF3). TSPAN8 is a protein that spans the membrane four times with transmembrane segments and has intracellular N- and C-termini. TSPAN8 has a small and a large extracellular loop that can mediate dimerization and association with other molecules including integrins, proteases, or cell signaling receptors. TSPAN8 is variably expressed on hematopoietic progenitor cells, spermatogonial stem cells, epithelial cells, and some tumor cells. TSPAN8 is involved in epithelial wound healing and may contribute to tumor cell metastasis.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.