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BV421 Rat Anti-Mouse TSPAN8
BV421 Rat Anti-Mouse TSPAN8

Flow cytometric analysis of TSPAN8 expression on mouse  polyploid carcinoma cells. CMT-93  cells (ATCC CCL-223) were detached using trypsin and then stained with either BD Horizon™ BV421 Rat IgG2b, κ Isotype Control (Cat. No. 562603; dashed line histogram) or BD OptiBuild™ BV421 Rat Anti-Mouse TSPAN8 (Cat. No. 748036; solid line histogram) at 1.0 µg/test. The fluorescence histograms showing TSPAN8 [or Ig Isotype control] staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

 

The above is qualification data only and does not represent a specific OptiBuild™ lot.

Flow cytometric analysis of TSPAN8 expression on mouse  polyploid carcinoma cells. CMT-93  cells (ATCC CCL-223) were detached using trypsin and then stained with either BD Horizon™ BV421 Rat IgG2b, κ Isotype Control (Cat. No. 562603; dashed line histogram) or BD OptiBuild™ BV421 Rat Anti-Mouse TSPAN8 (Cat. No. 748036; solid line histogram) at 1.0 µg/test. The fluorescence histograms showing TSPAN8 [or Ig Isotype control] staining were derived from gated events with the forward and side light-scatter characteristics of viable singlet cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software.

 

The above is qualification data only and does not represent a specific OptiBuild™ lot.

Product Details
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BD OptiBuild™
tetraspanin-8; tspan-8; Tm4sf3; Tspan8
Mouse (Tested in Development)
Rat IgG2b, κ
Mouse Tspan8 Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
AB_2872497
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment.  Fluorescent dye interactions may cause staining artifacts which may affect data interpretation.  The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions.  More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

  1. This antibody was developed for use in flow cytometry.
  2. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  3. Researchers should determine the optimal concentration of this reagent for their individual applications.
  4. An isotype control should be used at the same concentration as the antibody of interest.
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  8. BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
  9. BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
  10. Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
748036 Rev. 2
Antibody Details
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657909

The 657909 monoclonal antibody specifically recognizes Tetraspanin-8 (TSPAN8) that belongs to the Transmembrane 4 (Tetraspanin) superfamily (TM4SF). This molecule is also known as Transmembrane 4 superfamily member 3 (TM4SF3). TSPAN8 is a protein that spans the membrane four times with transmembrane segments and has intracellular N- and C-termini. TSPAN8 has a small and a large extracellular loop that can mediate dimerization and association with other molecules including integrins, proteases, or cell signaling receptors. TSPAN8 is variably expressed on hematopoietic progenitor cells, spermatogonial stem cells, epithelial cells, and some tumor cells.  TSPAN8 is involved in epithelial wound healing and may contribute to tumor cell metastasis.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.

748036 Rev. 2
Format Details
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BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
BV421
Violet 405 nm
407 nm
423 nm
748036 Rev.2
Citations & References
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Development References (4)

  1. Fu NY, Rios AC, Pal B, et al. Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive.. Nat Cell Biol. 2017; 19(3):164-176. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  2. Lu J, Izvolsky KI, Qian J, Cardoso WV. Identification of FGF10 targets in the embryonic lung epithelium during bud morphogenesis. J Biol Chem. 2005; 280(6):4834-4841. (Biology). View Reference
  3. Mutoji K, Singh A, Nguyen T, et al. TSPAN8 Expression Distinguishes Spermatogonial Stem Cells in the Prepubertal Mouse Testis. Biol Reprod. 2016; 95(6):117. (Clone-specific: Flow cytometry, Fluorescence activated cell sorting). View Reference
  4. Yue S, Zhao K, Erb U, Rana S, Zoller M. Joint features and complementarities of Tspan8 and CD151 revealed in knockdown and knockout models. Biochem Soc Trans. 2017; 45(2):437-447. (Biology). View Reference
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748036 Rev. 2

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.