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BD Horizon™ BV421 Rat Anti-Mouse LPAM-1
Clone DATK32 (RUO)
Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562302; Left Plot) or BD Horizon BV421 Rat Anti-Mouse LPAM-1 antibody (Cat. No. 562943/566294; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562302; Left Plot) or BD Horizon BV421 Rat Anti-Mouse LPAM-1 antibody (Cat. No. 562943/566294; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562302; Left Plot) or BD Horizon BV421 Rat Anti-Mouse LPAM-1 antibody (Cat. No. 562943/566294; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Multicolor flow cytometric analysis of LPAM-1 expression on C57BL/6 mouse bone marrow cells. Bone marrow cells were stained with APC Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 553092/561880) and with either BD Horizon™ BV421 Rat IgG2a, κ Isotype Control (Cat. No. 562302; Left Plot) or BD Horizon BV421 Rat Anti-Mouse LPAM-1 antibody (Cat. No. 562943/566294; Right Plot). Two-color flow cytometric dot plots show the correlated expression patterns of LPAM-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of viable bone marrow cells. Flow cytometry was performed using a BD™ LSR II Flow Cytometer System.
Product Details
BD Horizon™
Itga4Itgb7; α4β7 Integrin; alpha 4 beta 7 integrin; CD49dβ7
Mouse (QC Testing)
Rat F344, also known as Fischer, CDF IgG2a, κ
AKR/Cum mouse T lymphoma line TK1
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_2739667
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV421 under optimum conditions, and unconjugated antibody and free BD Horizon BV421 were removed.
Recommended Assay Procedures
For optimal and reproducible results, BD Horizon Brilliant™ Stain Buffer should be used anytime BD Horizon Brilliant™ dyes are used in a multicolor flow cytometry panel. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. When BD Horizon Brilliant Stain Buffer is used in in the multicolor panel, it should also be used in the corresponding compensation controls for all dyes to achieve the most accurate compensation. For the most accurate compensation, compensation controls created with either cells or beads should be exposed to BD Horizon Brilliant Stain Buffer for the same length of time as the corresponding multicolor panel. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794/566349) or the BD Horizon Brilliant Stain Buffer Plus (Cat. No. 566385).
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- BD Horizon Brilliant Violet 421 is covered by one or more of the following US patents: 8,158,444; 8,362,193; 8,575,303; 8,354,239.
- BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
Brilliant Stain Buffer RUO
Size
100 Tests
Cat No.
563794
BV421 Rat IgG2a, κ Isotype Control RUO
Size
50 µg
Cat No.
562602
APC Rat Anti-Mouse CD45R/B220 RUO
Size
0.1 mg
Cat No.
553092
APC Rat Anti-Mouse CD45R/B220 RUO
Size
25 mg
Cat No.
561880
566294 Rev. 2
Antibody Details
DATK32
The DATK32 monoclonal antibody specifically binds to an epitope specific for the α4β7 integrin heterodimer. α4β7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells. It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1. DATK32 antibody induces α4β7-dependent lymphocyte aggregation, but it inhibits other α4β7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).
566294 Rev. 2
Format Details
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
407 nm
423 nm
566294 Rev.2
Citations & References
View product citations for antibody "566294" on CiteAb
Development References (5)
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Andrew DP, Berlin C, Honda S, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol. 1994; 153(9):3847-3861. (Immunogen: Blocking, Induction). View Reference
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Andrew DP, Rott LS, Kilshaw PJ, Butcher EC. Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes. Eur J Immunol. 1996; 26(4):897-905. (Clone-specific: Blocking). View Reference
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Berlin C, Berg EL, Briskin MJ, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell. 1993; 74(1):185-195. (Clone-specific: Blocking). View Reference
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Hamann A, Andrew DP, Jablonski-Westrich D, Holzmann B, Butcher EC. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol. 1994; 152(7):3282-3293. (Clone-specific). View Reference
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Kilshaw PJ, Murant SJ. Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system. Eur J Immunol. 1991; 21(10):2591-2597. (Biology). View Reference
566294 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.