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BV421 Mouse Anti-Human CD314 (NKG2D)
Two-color flow cytometric analysis of CD314 (NKG2D) expression on human peripheral blood lymphocytes. Human whole blood was stained with PE Anti-Human CD56 (Cat. No. 555516) and with either BD Horizon™ BV421 Mouse IgG1, κ Isotype Control (Cat. No. 562438; Left Plot) or BD Horizon™ BV421 Mouse Anti-Human CD314 (NKG2D) antibody (Cat. No. 568106; Right Plot) at 0.5 µg/test. The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). A bivariate pseudocolor density plot showing the correlated expression of CD314 (NKG2D) [or Ig Isotype control staining] versus CD56 was derived from gated events with the forward and side light-scatter characteristics of intact lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ BV421 Mouse Anti-Human CD314 (NKG2D)
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