The 6B1monoclonal antibody specifically binds to TREM-1 (Triggering Receptor Expressed on Myeloid Cells 1 or Triggering Receptor Expressed on Monocytes 1) which is also known as CD354. CD354 is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. CD354 is highly expressed by neutrophils and monocytes. It is also reportedly expressed at lower levels by a variety of cells including subsets of T and B cells, NK cells and dendritic cells. Its expression is upregulated on leucocytes in response to microbial lipopolysaccharide, eg, during microbial infections or sepsis. CD354 activation triggers the release of proinflammatory cytokines and chemokines. A soluble form of CD354 has been described that may play a protective role in some infectious disease states.
The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor® 700-like dyes (eg, 712/20-nm filter).
Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents. Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended. Different BUV737 reagents (eg, CD4 vs. CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.