The 34-5-3 antibody reacts with the β chain of the I-A[d] MHC class II alloantigen. It cross-reacts with I-A[b] and with cells from mice of the H-2[p] and H-2[q] haplotypes. Reactivity with other haplotypes (e.g., a, f, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar to that of anti-I-A[b] mAb 25-9-17 (Cat. Nos. 553603, 553604, and 553605).
The antibody was conjugated to BD Horizon BUV615 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome with an Ex Max near 350 nm and an Em Max near 615 nm. BD Horizon Brilliant BUV615 can be excited by the ultraviolet laser (355 nm) and detected with a 610/20 filter and a 595 nm LP. Due to the excitation of the acceptor dye by the blue/yellow-green laser line, there may be significant spillover into channels detecting PE-CF594 like emissions (eg, 610/20-nm filter).