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BUV563 Mouse Anti-Human ICOS (CD278)
Flow cytometric analysis of ICOS (CD278) expression on stimulated human peripheral blood lymphocytes. Phytohemagglutinin (PHA)-stimulated (3 days) peripheral blood mononuclear cells were stained with either BD Horizon™ BUV563 Mouse IgG1 κ Isotype Control (Cat. No. 612920; dashed line histogram) or BD Horizon™ BUV563 Mouse Anti-Human ICOS (CD278) antibody (Cat. No. 568215; solid line histogram) at 0.25 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The fluorescence histogram showing ICOS (CD278) expression (or Ig Isotype control staining) was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
BD Horizon™ BUV563 Mouse Anti-Human ICOS (CD278)
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