This antibody is specific for mouse IgG1 of the Igh-C[a] and the c, d, e, f, g, h, n, o, and p haplotypes (e.g. BALB/c, C58, A, AKR, CBA, C3H, DBA/1, DBA/2, NZB, SWR). It has been reported not to react with IgG1 of the Igh-C[b] haplotype (e.g. C57BL/6, C57BL/10, SJL).
The antibody was conjugated to BD Horizon™ BUV496 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 496-nm. BD Horizon BUV496 can be excited by the ultraviolet laser (355 nm) and detected with a 515/30 nm filter with a 450LP. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into the channel detecting BD Horizon V500 or BV510 (eg, 525/40-nm filter). However, the spillover can be corrected through compensation as with any other dye combination.