Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
The SAM.2.rMAb is a recombinant monoclonal antibody that recognizes TCR Cβ2 expressed by a large proportion of CD4+ and CD8+ T cells. Thymocytes and mature peripheral T cells predominantly express a heterodimeric T cell receptor (TCR αβ) for antigen which is comprised of disulfide-liked transmembrane α and β chain subunits. The constant region of the TCR α subunit is encoded by TRAC, whereas the TCR β subunit is encoded by either of two highly homologous constant region genes, TCRB1 for TCR Cβ1 or TCRB2 for TCR Cβ2. The JOVI.1 antibody alternatively recognizes TCR Cβ1 expressed by the other TCR αβ+ T cells. These antibodies are effectively used together in multicolor staining and flow cytometric analyses to identify and characterize the natures of either TCR Cβ1+ or TCR Cβ2+ T cells in heterogeneous cell populations.
Note - Some human CD3- and TCR-specific antibodies might not be compatible to co-stain human T cells with SAM2.rMab. To confirm compatibility and immunofluorescent staining protocols, please visit: https://www.bdbiosciences.com/content/dam/bdb/marketing-documents/discover-learn/thought-leadership/events/POSTER-AAI%20202024-S-Riguad.pdf
Development References (7)
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Berg H, Otteson GE, Corley H, et al. Flow cytometric evaluation of TRBC1 expression in tissue specimens and body fluids is a novel and specific method for assessment of T-cell clonality and diagnosis of T-cell neoplasms.. Cytometry B Clin Cytom. 2021; 100(3):361-369. (Biology). View Reference
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Ferrari M, Baldan V, Ghongane P, et al. Targeting TRBC1 and 2 for the treatment of T cell lymphomas. Abstract. Cancer Res. 2020; 80:2183. (Biology).
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Horna P, Shi M, Olteanu H, Johansson U. Emerging Role of T-cell Receptor Constant β Chain-1 (TRBC1) Expression in the Flow Cytometric Diagnosis of T-cell Malignancies.. Int J Mol Sci. 2021; 22(4):1817. (Biology). View Reference
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Maciocia PM, Wawrzyniecka PA, Philip B, et al. Targeting the T cell receptor β-chain constant region for immunotherapy of T cell malignancies.. Nat Med. 2017; 23(12):1416-1423. (Biology). View Reference
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Morath A, Schamel WW. αβ and γδ T cell receptors: Similar but different.. J Leukoc Biol. 2020; 107(6):1045-1055. (Biology). View Reference
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Muñoz-García N, Lima M, Villamor N, et al. Anti-TRBC1 Antibody-Based Flow Cytometric Detection of T-Cell Clonality: Standardization of Sample Preparation and Diagnostic Implementation.. Cancers (Basel). 2021; 13(17):4379. (Biology). View Reference
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Tesio M. Subtle Differences to Make the Difference.. Hemasphere. 2018; 2(2):e38. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.