BD Pharmingen™ Alexa Fluor™ 647 Mouse Anti-Human CD319 (CRACC)
Clone 162/CRACC (also known as 162)
(RUO)
Multiparameter flow cytometric analysis of CD319 (CRACC) expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 563798) and with either Alexa Fluor™ 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plots) or Alexa Fluor™ 647 Mouse Anti-Human CD319 (CRACC) antibody (Cat. No. 570756/570834; Right Plots). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD319 (CRACC) [or Ig Isotype control staining] versus side light-scatter signals were derived from gated events with the forward and side light scatter characteristics of intact leukocyte populations (Top Plots). The bivariate plots showing the correlated expression of CD319 (CRACC) [or Ig Isotype control staining] versus CD3 were derived from gated events with the forward and side light scatter characteristics of intact lymphocytes (Bottom Plots). Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.
Multiparameter flow cytometric analysis of CD319 (CRACC) expression on Human peripheral blood leukocytes. Human whole blood was stained with BD Horizon™ BV421 Mouse Anti-Human CD3 antibody (Cat. No. 563798) and with either Alexa Fluor™ 647 Mouse IgG2b, κ Isotype Control (Cat. No. 565378; Left Plots) or Alexa Fluor™ 647 Mouse Anti-Human CD319 (CRACC) antibody (Cat. No. 570756/570834; Right Plots). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Bivariate pseudocolor density plots showing the correlated expression of CD319 (CRACC) [or Ig Isotype control staining] versus side light-scatter signals were derived from gated events with the forward and side light scatter characteristics of intact leukocyte populations (Top Plots). The bivariate plots showing the correlated expression of CD319 (CRACC) [or Ig Isotype control staining] versus CD3 were derived from gated events with the forward and side light scatter characteristics of intact lymphocytes (Bottom Plots). Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ Software.
Product Details
BD Pharmingen™
19A; 19A24 protein; CD319; CRACC; CS1; SLAMF7
Human (QC Testing)
Mouse BALB/c IgG2b, κ
Human extracellular CRACC
Flow cytometry (Routinely Tested)
5 µl/test
VIII 80452
57823
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Alexa Fluor™ is a trademark of Life Technologies Corporation.
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control RUO
Size
50 µg
Cat No.
565378
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
BV421 Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
563798
570756 Rev. 1
Antibody Details
162/CRACC
The 162 monoclonal antibody specifically binds to CRACC (CD2-like receptor activating cytotoxic cells) which is also known as CD319, CS1 (CD2 subset 1), 19A, or 19A24. CD319 (CRACC) is a ~66 kDa type I transmembrane glycoprotein that is encoded by SLAMF7 (SLAM family member 7) which belongs to the CD2 family within the Ig superfamily. CD319 (CRACC) is expressed on most NK cells and subsets of CD8+ T cells, CD4+ T cells, B cells, activated macrophages, and dendritic cells. CRACC reportedly plays roles in the activation, proliferation and effector functions of T cells, NK cells, and other leucocytes.
570756 Rev. 1
Format Details
Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
570756 Rev.1
Citations & References
View product citations for antibody "570756" on CiteAb
Development References (6)
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Boles KS, Mathew PA. Molecular cloning of CS1, a novel human natural killer cell receptor belonging to the CD2 subset of the immunoglobulin superfamily. Immunogenetics. 2001; 52(3-4):302-307. (Biology). View Reference
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Bouchon A, Cella M, Grierson HL, Cohen JI, Colonna M. Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family. J Immunol. 2001; 167(10):5517-5521. (Immunogen: Flow cytometry, Functional assay, Immunoprecipitation). View Reference
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Hagberg N, Theorell J, Schlums H, Eloranta ML, Bryceson YT, Ronnblom L. Systemic lupus erythematosus immune complexes increase the expression of SLAM family members CD319 (CRACC) and CD229 (LY-9) on plasmacytoid dendritic cells and CD319 on CD56(dim) NK cells. J Immunol. 2013; 191(6):2989-2998. (Clone-specific). View Reference
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Kim JR, Horton NC, Mathew SO, Mathew PA. CS1 (SLAMF7) inhibits production of proinflammatory cytokines by activated monocytes. Inflamm Res. 2013; 62(8):765-772. (Biology). View Reference
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Murphy JJ, Hobby P, Vilarino-Varela J, et al. A novel immunoglobulin superfamily receptor (19A) related to CD2 is expressed on activated lymphocytes and promotes homotypic B-cell adhesion. Biochem J. 2002; 361(Pt3):431-436. (Biology). View Reference
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Tassi I, Colonna M. The cytotoxicity receptor CRACC (CS-1) recruits EAT-2 and activates the PI3K and phospholipase Cgamma signaling pathways in human NK cells. J Immunol. 2005; 175(12):7996-8002. (Clone-specific: Flow cytometry, Functional assay). View Reference
570756 Rev. 1
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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.