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Alexa Fluor® 647 Mouse anti-FAK (pS910)
Alexa Fluor® 647 Mouse anti-FAK (pS910)

Analysis of FAK (pS910) in human epithelioid carcinoma.  Hela S3 cells (ATCC CCL 2.2) were either stimulated with Nocodazole at 37°C for 16 hours (shaded histogram) or unstimulated (open histogram).  The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37°C, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, then stained with Alexa Fluor® 647  Mouse anti-FAK (pS910 Cat. No. 558545).  Flow cytometry was performed on a BD FACSCalibur™  flow cytometry system.

Analysis of FAK (pS910) in human epithelioid carcinoma.  Hela S3 cells (ATCC CCL 2.2) were either stimulated with Nocodazole at 37°C for 16 hours (shaded histogram) or unstimulated (open histogram).  The cells were fixed (BD Cytofix™ buffer, Cat. No. 554655) for 10 minutes at 37°C, then permeabilized (BD Phosflow™ Perm Buffer III, Cat. No. 558050) on ice for at least 30 minutes, then stained with Alexa Fluor® 647  Mouse anti-FAK (pS910 Cat. No. 558545).  Flow cytometry was performed on a BD FACSCalibur™  flow cytometry system.

Product Details
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BD Phosflow™
Human (QC Testing), Mouse, Rat (Predicted)
Mouse BALB/c IgG2b, κ
Phosphorylated Human FAK
Intracellular staining (flow cytometry) (Routinely Tested)
20 µl
AB_647127
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558545 Rev. 2
Antibody Details
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K73-480

Focal Adhesion Kinase (FAK) is a cytoplasmic tyrosine kinase that associates with integrins in focal adhesions.  Its cellular localization is directed by a 125-amino acid sequence at the C terminus called the "Focal Adhesion Targeting" (FAT) domain, and the phosphorylation state of serine 910 (S910) in the FAT domain may regulate the assembly of focal adhesions.  Furthermore, the binding of extracellular matrix ligands to integrins triggers tyrosine phosphorylations near FAK's kinase domain that increase its kinase activity, and additional tyrosine phosphorylations near proline-rich motifs create binding sites for the SH2 domains of various adaptor proteins.  FAK's ability to bind numerous structural and signaling proteins via a variety of interactions regulates FAK's targeting to focal adhesions, modulates its kinase activity, and initiates intracellular signaling cascades.  Thus, studies suggest that FAK may integrate cellular events controlling cell motility, growth, and invasiveness.  

The K73-480 monoclonal antibody recognizes the phosphorylated S910 of human FAK.  The orthologous phosphorylation sites in mouse and rat FAK are S948 and S913, respectively.

558545 Rev. 2
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
558545 Rev.2
Citations & References
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Development References (4)

  1. Hunger-Glaser I, Perez Salazar E, Sinnett-Smith J, Rozengurt E. Bombesin, lysophosphatidic acid, and epidermal growth factor rapidly stimulate focal adhesion kinase phosphorylation at Ser-910. Requirement for ERK activation. J Biol Chem. 2003; 278(25):22631-22643. (Biology).
  2. Ma A, Richardson A, Schaefer EM, Parsons JT. Serine phosphorylation of focal adhesion kinase in interphase and mitosis: A possible role in modulating binding to p130Cas. Mol Biol Cell. 2001; 12:1-12. (Biology). View Reference
  3. Schlaepfer DD, Mitra SK, Ilic D. Control of motile and invasive cell phenotypes by focal adhesion kinase. Biochim Biophys Acta. 2004; 1692:77-102. (Biology). View Reference
  4. Yamakita Y, Totsukawa G, Yamashiro S, et al. Dissociation of FAK/c-Src complex during mitosis: Role of mitosis-specific serine phosphorylation of FAK. J Cell Biol. 1999; 144(2):315-324. (Biology). View Reference
View All (4) View Less
558545 Rev. 2

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.