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Alexa Fluor™ 488 Rat Anti-Mouse CD169 (Siglec-1)

BD Pharmingen™ Alexa Fluor™ 488 Rat Anti-Mouse CD169 (Siglec-1)

Clone 3D6/CD169 (also known as 3D6)

(RUO)
Alexa Fluor™ 488 Rat Anti-Mouse CD169 (Siglec-1)
Multicolor flow cytometric analysis of CD169 (Siglec -1) expression on Mouse myeloid cells.  C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either Alexa Fluor™ 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676; Left Plot) or Alexa Fluor™ 488 Rat Anti-Mouse CD169 (Siglec-1) antibody (Cat. No. 570768/570846; Right Plot) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 (Siglec-1) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of CD169 (Siglec -1) expression on Mouse myeloid cells.  C57BL/6 Mouse bone marrow cells were preincubated with Purified Rat Anti-Mouse CD16/CD32 antibody (Mouse BD Fc Block™) [Cat. No. 553141/553142]. The cells were then stained with BD Horizon™ BV421 Rat Anti-Mouse Ly-6G and Ly-6C antibody (Cat. No. 562709) and with either Alexa Fluor™ 488 Rat IgG2a, κ Isotype Control (Cat. No. 557676; Left Plot) or Alexa Fluor™ 488 Rat Anti-Mouse CD169 (Siglec-1) antibody (Cat. No. 570768/570846; Right Plot) at 1 µg/test. BD Via-Probe™ Cell Viability 7-AAD Solution (Cat. No. 555815/555816) was added to cells right before analysis. The bivariate pseudocolor density plot showing the correlated expression of CD169 (Siglec-1) [or Ig Isotype control staining] versus Ly-6G and Ly-6C was derived from gated events with the forward and side light-scatter characteristics of viable (7-AAD-negative) bone marrow cells. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Flow Cytometer System and FlowJo™ Software. Data shown on this Technical Data Sheet are not lot specific.
Product Details
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BD Pharmingen™
Anti-mouse sialoadhesin
Mouse (QC Testing)
Rat AO IgG2a, κ
Purified sialoadhesin from mouse spleen
Flow cytometry (Routinely Tested)
0.2 mg/ml
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.  However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. An isotype control should be used at the same concentration as the antibody of interest.
  4. Alexa Fluor® 488 fluorochrome emission is collected at the same instrument settings as for fluorescein isothiocyanate (FITC).
  5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  7. This product is provided under an intellectual property license between Life Technologies Corporation and BD Businesses. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for any other use, contact Life Technologies Corporation, Cell Analysis Business Unit Business Development, 29851 Willow Creek Road, Eugene, OR 97402, USA, Tel: (541) 465-8300. Fax: (541) 335-0504.
  8. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  9. Alexa Fluor™ is a trademark of Life Technologies Corporation.
  10. For U.S. patents that may apply, see bd.com/patents.
570846 Rev. 1
Antibody Details
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3D6/CD169

The 3D6 monoclonal antibody specifically recognizes CD169 which is a ~185 kDa type I transmembrane glycoprotein that is encoded by Siglec1. CD169 belongs to the Sialic acid binding Ig like lectins (Siglec) family and is also known as Sialoadhesin (Sn), Siglec-1, or Sheep erythrocyte receptor (SER). CD169 is expressed on subsets of macrophages found in the bone marrow, secondary lymphoid tissues, liver colon and lungs. It binds to the oligosaccharide sequence NeuAc alpha 2,3Gal present on glycolipids and glycoproteins including CD43, CD162, CD206, or CD227. CD169 serves as an adhesion molecule that can mediate cellular interactions with the extracellular matrix or with other cells including lymphocytes and myeloid cells. CD169 may play a role in erythroid and myeloid cell development as well as leucocyte trafficking and inflammation.

570846 Rev. 1
Format Details
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Alexa Fluor™ 488
Alexa Fluor™ 488 Dye is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 517-nm. Alexa Fluor™ 488 is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 520-nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 488
Blue 488 nm
494 nm
517 nm
570846 Rev.1
Citations & References
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View product citations for antibody "570846" on CiteAb

Development References (4)

  1. Crocker PR, Freeman S, Gordon S, Kelm S. Sialoadhesin binds preferentially to cells of the granulocytic lineage.. J Clin Invest. 1995; 95(2):635-43. (Immunogen: Blocking, Radioimmunoassay). View Reference
  2. Ducreux J, Crocker PR, Vanbever R. Analysis of sialoadhesin expression on mouse alveolar macrophages.. Immunol Lett. 2009; 124(2):77-80. (Clone-specific: Blocking, Inhibition). View Reference
  3. Kumamoto Y, Higashi N, Denda-Nagai K, et al. Identification of sialoadhesin as a dominant lymph node counter-receptor for mouse macrophage galactose-type C-type lectin 1.. J Biol Chem. 2004; 279(47):49274-80. (Clone-specific: Fluorescence microscopy, Immunofluorescence, Western blot). View Reference
  4. Nath D, van der Merwe PA, Kelm S, Bradfield P, Crocker PR. The amino-terminal immunoglobulin-like domain of sialoadhesin contains the sialic acid binding site. Comparison with CD22.. J Biol Chem. 1995; 270(44):26184-91. (Clone-specific: Functional assay, Immunoaffinity chromatography). View Reference
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570846 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.