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Human and Mouse Pluripotent Stem Cell Analysis Kit

Human and Mouse Pluripotent Stem Cell Analysis Kit

(RUO)
Product Details
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BD Stemflow™
Human,Mouse (QC Testing), Rhesus (Reported)
RUO
AB_2869350


Description

Preparation And Storage

The entire BD Stemflow Human and Mouse Pluripotent Stem Cell Analysis Kit must be stored in the dark at 2° to 8°C. Do not freeze.

560477 Rev. 1
Components
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Description Quantity/Size Part Number
Alexa Fluor® 647 Rat anti-SSEA-4 1.5 mL (1 ea) 51-9006265
PerCP-Cy™5.5 Mouse anti-Oct3/4 1.5 mL (1 ea) 51-9006267
PE Rat anti-SSEA-1 1.5 mL (1 ea) 51-9006268
Alexa 647 Mouse IgG3, κ Isotype Control N/A 51-9006270
PerCP-Cy™5.5 Mouse IgG1, κ Isotype Control N/A 51-9006272
PE Mouse IgM, κ Isotype Control N/A 51-9006273
Negative Control (PBS with 1% BSA) 50 Tests (1 ea) 51-9006227
Anti-Mouse Ig, κ 6 mL (1 ea) 51-9006274
Perm/Wash Buffer N/A 51-9006275
Fixation Buffer 50 mL (1 ea) 51-9006276
560477 Rev. 1
Citations & References
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Development References (10)

  1. Draper JS, Pigott C, Thomson JA, Andrews PW. Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat. 2002; 200:249-258. (Biology). View Reference
  2. Ginis I, Luo Y, Miura T, et al. Differences between human and mouse embryonic stem cells. Dev Biol. 2004; 269:360-380. (Biology).
  3. Henderson JK, Draper JS, Baillie HS, et al. Preimplantation human embryos and embryonic stem cells show comparable expression of stagespecific embryonic antigens. Stem Cells. 2002; 20:329-337. (Biology).
  4. International Stem Cell Initiative. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat Biotechnol. 2007; 25(7):803-816. (Biology). View Reference
  5. Lowry WE, Richter L, Yachechko R, et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A. 2008; 105:2883-2888. (Biology).
  6. Okamoto K, Okazawa H, Okuda A, Sakai M, Muramatsu M, Hamada H. A novel octamer binding transcription factor is differentially expressed in mouse embryonic cells. Cell. 1990; 60(3):461-472. (Biology). View Reference
  7. Park IH, Zhao R, West JA, et al. Reprogramming of human somatic cells to pluripotency with defined factors. Nature. 2008; 451:141-146. (Biology).
  8. Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat Biotechnol. 2000; 18:399-404. (Biology).
  9. Solter D, Shevinsky L, Knowles BB, Strickland S. The induction of antigenic changes in a teratocarcinoma stem cell line (F9) by retinoic acid. Dev Biol. 1979; 70:515-521. (Biology).
  10. Xu C. Characterization and evaluation of human embryonic stem cells. Methods Enzymol. 2006; 420:18–37. (Biology).
View All (10) View Less
560477 Rev. 1

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.