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      PerCP-Cy™5.5 Mouse Anti-Human β2-Microglobulin
      Product Details
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      BD™
      Human
      Mouse IgM, κ
      Flow cytometry
      25 μg/mL
      20 μL
      N/A
      567
      AB_2870412
      RUO (GMP)


      656645 Rev. 1
      Antibody Details
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      TÜ99

      The β-Microglobulin antibody, clone TÜ99, is derived from the hybridization of P3-NS1/1-Ag4-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with a human uncloned primed lymphocyte typing (PLT) cell line.

      The β-Microglobulin antibody specifically recognizes β-microglobulin, a 12-kilodalton (kDa) protein with homology to the constant region of immunoglobulin molecules.

      656645 Rev. 1
      Format Details
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      PerCP-Cy5.5
      PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      PerCP-Cy5.5
      482 nm
      676 nm
      656645 Rev.1
      Citations & References
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      View product citations for antibody "656645" on CiteAb

      Development References (15)

      1. Bix M, Coles M, Raulet D. Positive selection of Vβ8+CD4-8- thymocytes by class I molecules expressed by hematopoietic cells. J Exp Med. 1993; 178:901-908. (Biology).
      2. Bix M, Raulet D. Functionally conformed free class I heavy chains exist on the surface of β 2 microglobulin negative cells. J Exp Med. 1992; 176:829-834. (Biology).
      3. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
      4. Clinical and Laboratory Standards Institute. 2005. (Biology).
      5. Grey HM, Kubo RT, Colon SM, et al. The small subunit of HL-A antigens is β 2-microglobulin. J Exp Med. 1973; 138:1608-1612. (Biology).
      6. Hicklin DJ, Wang Z, Arienti F, Rivoltini L, Parmiani G, Ferrone S. β2-Microglobulin mutations, HLA class I antigen loss, and tumor progression in melanoma. J Clin Invest. 1998; 101:2720-2729. (Biology).
      7. Koller BH, Marrack P, Kappler JW, Smithies O. Normal development of mice deficient in β2M, MHC class I proteins, and CD8+ T cells. Science. 1990; 248:1227-1230. (Biology).
      8. Martin D, Fauchet R, Müller C, et al. Expression of HLA-A and -B antigens on differentiating U-937 cells. Tissue Antigens. 1985; 25:235-246. (Biology).
      9. Peterson PA, Cunningham BA, Berggård I, Edelman GM. β2</sub-Microglobulin—a free immunoglobulin domain. Proc Natl Acad Sci USA. 1972; 69:1697-1701. (Biology).
      10. Peterson PA, Rask L, Lindblom JB. Highly purified papain-solubilized HL-A antigens contain β2-microglobulin. Proc Natl Acad Sci USA. 1974; 71:35-39. (Biology).
      11. Pérarnau B, Siegrist CA, Gillet A, Vincent C, Kimura S, Lemonnier FA. β 2-microglobulin restriction of antigen presentation. Nature. 1990; 346:751-754. (Biology).
      12. Vitiello A, Potter TA, Sherman LA. The role of β 2-microglobulin in peptide binding by class I molecules. Science. 1990; 250:1423-1426. (Biology).
      13. Wang Z, Arienti F, Parmiani G, Ferrone S. Induction and functional characterization of β2-microglobulin (β2-mu)-free HLA class I heavy chains expressed by β2-mu-deficient human FO-1 melanoma cells. Eur J Immunol. 1998; 28:2817-2826. (Biology).
      14. Zijlstra M, Bix M, Simister NE, Loring JM, Raulet DH, Jaenisch R. β2-Microglobulin deficient mice lack CD4-8+ cytolytic T cells. Nature. 1990; 344:742-746. (Biology).
      15. van Dongen JJ, Lhermitte L, Böttcher S, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9):1908-1975. (Biology). View Reference
      View All (15) View Less
      656645 Rev. 1

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures. 

       

      Although not required, these products are manufactured in accordance with Good Manufacturing Practices.