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PE Mouse Anti-Human IL-8
Product Details
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BD FastImmune™
Mouse BALB/c IgG1, κ
Flow cytometry
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.

Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340510 Rev. 1
Antibody Details
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Anti-Hu–IL-8, clone AS14, is derived from the hybridization of mouse P3X63Ag8 cells with spleen cells from BALB/c mice immunized with recombinant human IL-8.

Anti-Human Interleukin-8 (Anti-Hu–IL-8) recognizes a nonglycosylated protein of approximately 8 kilodaltons (kd).

340510 Rev. 1
Format Details
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R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340510 Rev.1
Citations & References
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Development References (35)

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  35. de Waal Malefyt R, Abrams J, Bennett B, et al. Interleukin 10 (IL-10) inhibits cytokine synthesis by human monocytes: An autoregulatory role of IL-10 produced by monocytes. J Exp Med. 1991; 174:1209-1220. (Biology).
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340510 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.