Skip to main content Skip to navigation
PE Mouse Anti-Human IL-1β
Product Details
Down Arrow Up Arrow


BD FastImmune™
IL-1 beta
Human
Mouse IgG1, κ
Human IL-1β
Flow cytometry
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO_GMP


Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340516 Rev. 1
Antibody Details
Down Arrow Up Arrow
AS10

Anti-Hu–IL-1β, clone AS10, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-1β.

Anti-Human Interleukin-1β (Anti-Hu–Il-1β) recognizes a 13- to 17-kilodalton (kd) polypeptide.

340516 Rev. 1
Format Details
Down Arrow Up Arrow
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
340516 Rev.1
Citations & References
Down Arrow Up Arrow

Development References (11)

  1. Conlon PJ, Grabstein KH, Alpert A, et al. Localization of human mononuclear cell interleukin 1. J Immunol. 1987; 139:98-102. (Biology).
  2. Dinarello CA. Biology of interleukin 1. FASEB J. 1988; 2:108-115. (Biology).
  3. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
  4. Giri JG, Lomedico PT, Mizel SB. Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1. J Immunol. 1985; 134(1):343-349. (Biology). View Reference
  5. Mantovani A, Dejana E. Cytokines as communication signals between leukocytes and endothelial cells. Immunol Today. 1989; 10:370-375. (Biology).
  6. Matsushima K, Yodoi J, Tagaya Y, Oppenheim JJ. Downregulation of interleukin-1 receptor expression by IL-1 and fate of internalized125 I-labeled IL-1 β in a human large granular lymphocyte cell line. J Immunol. 1986; 137:3183-3188. (Biology).
  7. Mizel SB. The interleukins. FASEB J. 1989; 32379-2388. (Biology).
  8. Saklatvala J. Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Science. 1986; 322:547-549. (Biology).
  9. Singer II, Scott S, Chin J, et al. The Interleukin-1 β –converting enzyme (ICE) is localized on the external cell surface membranes and in the cytoplasmic ground substance of human monocytes by immuno-electron microscopy. J Exp Med. 1995; 182:1447-1459. (Biology).
  10. Slack J, McMahan CJ, Waugh S. et al. J Bio Chem. 1989; 10:370-375. (Biology).
  11. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
View All (11) View Less
340516 Rev. 1

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.