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FITC Mouse Anti-Human IL-1β
Product Details
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BD FastImmune™
IL-1 beta
Human
Mouse IgG1, κ
Human IL-1β
Intracellular staining (flow cytometry)
20 μL
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO_GMP


Preparation And Storage

The FITC and PE conjugates are each supplied in 1.0 mL of PBS containing gelatin and 0.1% sodium azide. Please refer to the vial label for antibody concentration. The vial should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

340515 Rev. 1
Antibody Details
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AS10

Anti-Hu–IL-1β, clone AS10, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-1β.

Anti-Human Interleukin-1β (Anti-Hu–Il-1β) recognizes a 13- to 17-kilodalton (kd) polypeptide.

340515 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
340515 Rev.1
Citations & References
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Development References (11)

  1. Conlon PJ, Grabstein KH, Alpert A, et al. Localization of human mononuclear cell interleukin 1. J Immunol. 1987; 139:98-102. (Biology).
  2. Dinarello CA. Biology of interleukin 1. FASEB J. 1988; 2:108-115. (Biology).
  3. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
  4. Giri JG, Lomedico PT, Mizel SB. Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1. J Immunol. 1985; 134(1):343-349. (Biology). View Reference
  5. Mantovani A, Dejana E. Cytokines as communication signals between leukocytes and endothelial cells. Immunol Today. 1989; 10:370-375. (Biology).
  6. Matsushima K, Yodoi J, Tagaya Y, Oppenheim JJ. Downregulation of interleukin-1 receptor expression by IL-1 and fate of internalized125 I-labeled IL-1 β in a human large granular lymphocyte cell line. J Immunol. 1986; 137:3183-3188. (Biology).
  7. Mizel SB. The interleukins. FASEB J. 1989; 32379-2388. (Biology).
  8. Saklatvala J. Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Science. 1986; 322:547-549. (Biology).
  9. Singer II, Scott S, Chin J, et al. The Interleukin-1 β –converting enzyme (ICE) is localized on the external cell surface membranes and in the cytoplasmic ground substance of human monocytes by immuno-electron microscopy. J Exp Med. 1995; 182:1447-1459. (Biology).
  10. Slack J, McMahan CJ, Waugh S. et al. Independent binding of interleukin-1 α and interleukin-1 β to type I and type II interleukin 1 receptors. J Bio Chem. 1993; 268:2513-2524. (Biology).
  11. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
View All (11) View Less
340515 Rev. 1

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.