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FITC Mouse Anti-Human IL-1α
Product Details
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BD FastImmune™
Human
Mouse BALB/c IgG1, κ
Human
Intracellular staining (flow cytometry)
6.3 μg/mL
20 μL
3552
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO_GMP


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

340513 Rev. 1
Antibody Details
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AS5

The Anti-Hu–IL-1α antibody, clone AS5, is derived from fusion of P3X63Ag8 myeloma cells with splenocytes from BALB/c mice immunized with recombinant human IL-1α.

The Anti-Human Interleukin-1α (Anti-Hu–IL-1α) antibody recognizes a 13- to 18-kilodalton (kDa) polypeptide.

340513 Rev. 1
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
340513 Rev.1
Citations & References
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Development References (14)

  1. Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Conlon PJ, Grabstein KH, Alpert A, et al. Localization of human mononuclear cell interleukin 1. J Immunol. 1987; 139:98-102. (Biology).
  4. Dinarello CA. Biology of interleukin 1. FASEB J. 1988; 2:108-115. (Biology).
  5. Dinarello CA. Interleukin-1 and interleukin-1 antagonism. Blood. 1991; 77(8):1627-1652. (Biology). View Reference
  6. Fini ME, Strissel KJ, Girard MT, Mays JW, Rinehart WB. Interleukin 1αmediates collegenase synthesis stimulated by Phorbol 12-Myristate 13-Acetate. J Bio Chem. 1994; 269:11291-11298. (Biology).
  7. Giri JG, Lomedico PT, Mizel SB. Studies on the synthesis and secretion of interleukin 1. I. A 33,000 molecular weight precursor for interleukin 1. J Immunol. 1985; 134(1):343-349. (Biology). View Reference
  8. Mantovani A, Dejana E. Cytokines as communication signals between leukocytes and endothelial cells. J Immunol. 1989; 10:370-375. (Biology).
  9. Matsushima K, Yodoi J, Tagaya Y, Oppenheim JJ. Downregulation of interleukin-1 receptor expression by IL-1 and fate of internalized125 I-labeled IL-1 β in a human large granular lymphocyte cell line. J Immunol. 1986; 137:3183-3188. (Biology).
  10. Mizel SB. The interleukins. FASEB J. 1989; 32379-2388. (Biology).
  11. Saklatvala J. Tumour necrosis factor α stimulates resorption and inhibits synthesis of proteoglycan in cartilage. Science. 1986; 322:547-549. (Biology).
  12. Slack J, McMahan CJ, Waugh S. et al. Independent binding of interleukin-1 α and interleukin-1 β to type I and type II interleukin 1 receptors. J Bio Chem. 1993; 268:2513-2524. (Biology).
  13. Van Zee KJ, DeForge LE, Fischer E, et al. IL-8 in septic shock, endotoxemia and after IL-1 administration. J Immunol. 1991; 146:3478-3482. (Biology).
  14. West-Mays JA, Strissel KJ, Sadow PM, Fini ME. Competence for collegenase gene expression by tissue fibroblasts requires activation of an interleukin 1α autocrine loop. Prod Natl Acad Sci. 1995; 92:6768-6772. (Biology).
View All (14) View Less
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