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APC Mouse Anti-Human IFN-γ
Product Details
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BD FastImmune™
Human
Mouse IgG2b
Recombinant human IFN-γ
Intracellular staining (flow cytometry)
12 μg/mL
5 μL
3458
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO_GMP


Preparation And Storage

The FITC conjugate is supplied as 12.5 μg in 1.0 mL (12.5 μg/mL) of PBS. The PE conjugate is supplied as 7.5 μg in 1.0 mL (7.5 μg/mL) of PBS. The APC conjugate is supplied as 6 μg in 0.5 mL (12 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide.

Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

341117 Rev. 1
Antibody Details
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25723.11

The anti-Hu–IFN-γ antibody, clone 25723.11, is derived from the hybridization of mouse P3X-63-Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.

The anti-human interferon-γ (anti-Hu–IFN-γ) antibody recognizes a 20- to 25-kilodalton (kDa) glycoprotein, which is a type II interferon. It is also known as immune interferon.

341117 Rev. 1
Format Details
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APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
APC
Red 627-640 nm
651 nm
660 nm
341117 Rev.1
Citations & References
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Development References (25)

  1. Aggarwal B, Puri R. Human Cytokines: Their Role in Disease and Therapy. Cambridge, MA: Blackwell Science; 1995:3-24.
  2. Alzona M, Jack H-M, Fisher RI, Ellis TM. CD30 defines a subset of activated human T cells that produce IFN-γ and IL-5 and exhibit B-cell helper activity. J Immunol. 1994; 153:2861-2867. (Biology).
  3. Carding SR, Hayday AC, Bottomly K. Cytokines in T-cell development. Immunol Today. 1991; 12:239-245. (Biology).
  4. Clerici M, Shearer GM. A TH1 → TH2 switch is a critical step in the etiology of HIV infection. Today. 1993; 14:107-110. (Biology).
  5. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
  6. ElGhazali GEB, Paulie S, Andersson G, et al. Number of interleukin-4– and interferon- γ –secreting human T cells reactive with tetanus toxoid and the mycobacterial antigen PPD or phytohemagglutindistinct response profiles depending on the type of antigen used for activation. Eur J Immunol. 1993; 23:2740-2745. (Biology).
  7. Elson LH, Nutman TB, Metcalfe DD, Prussin C. Flow cytometric analysis for cytokine production identifies T helper 1, T helper 2, and T helper 0 cells within the human CD4+ CD27- lymphocyte subpopulation. J Immunol. 1995; 154:4294-4301. (Biology).
  8. Fan J, Bass HZ, Fahey JL. Elevated IFN-γ and decreased IL-2 gene expression are associated with HIV infection. J Immunol. 1993; 151:5031-5040. (Biology).
  9. Ghanekar SA, Nomura LE, Suni MA, Picker LJ, Maecker HT, Maino VC. γ interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65. Clin Diagn Lab Immunol. 2001; 8:628-631. (Biology).
  10. Graziosi C, Pantaleo G, Gantt KR, et al. Lack of evidence for the dichotomy of T H 1 and T H 2 predominance in HIV-infected individuals. Science. 1994; 265:248-252. (Biology).
  11. Hardy KJ, Sawada T. Human γ interferon strongly upregulates its own gene expression in peripheral blood lymphocytes. J Exp Med. 1989; 170:1021-1026. (Biology).
  12. Hyjek E, Lischner HW, Hyslop T, et al. Cytokine patterns during progression to AIDS in children with perinatal HIV infection. J Immunol. 1995; 155:4060-4071. (Biology).
  13. Johnson HM, Bazer FW, Szente BE, Jarpe MA. How interferons fight disease. Scientific American. 1994; May:68-75. (Biology).
  14. Maecker HT, Auffermann-Gretzinger S, Nomura LE, et al. Detection of CD4 T-cell responses to a tumor vaccine by cytokine flow cytometry. Clin Cancer Res. 2001; 7:902s-908s. (Biology).
  15. Maggi E, Mazzetti M, Ravina A, et al. Ability of HIV to promote a TH1 to TH0 shift and to replicate preferentially in TH2 and TH 0 cells. Science. 1994; 265:244-248. (Biology).
  16. Maino VC, Maecker HT, Picker LJ. Cossarizza A, Kaplan D, ed. Cellular Aspects of HIV Infection. Wiley-Liss, Inc; 2002:371-382.
  17. Noble A, Macary PA, Kemeny DM. IFN-γ and IL-4 regulate the growth and differentiation of CD8 + T cells into subpopulations with distince cytokine profiles. J Immunol. 1995; 155:2928-2937. (Biology).
  18. Nomura LE, Walker JM, Maecker HT. Optimization of whole blood antigen-specific cytokine assays for CD4+ T cells. Cytometry. 2000; 40:60-68. (Biology).
  19. Openshaw P, Murphy EE, Hosken NA, et al. Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations. J Exp Med. 1995; 182(5):1357-1367. (Biology). View Reference
  20. Paliard X, Malefijt RDW, Yssel H, et al. Simultaneous production of IL-2, IL-4, and IFN-γ by activated human CD4+ and CD8+ T cell clones. J Immunol. 1988; 141:849-855. (Biology).
  21. Picker LJ, Singh MK, Zdraveski Z, et al. Direct demonstration of cytokine synthesis heterogeneity among human memory/effector T cells by flow cytometry. Blood. 1995; 86:1408-1419. (Biology).
  22. Powrie F, Coffman RL. Cytokine regulation of T-cell function: potential for therapeutic intervention. Immunol Today. 1993; 14:270-274. (Biology).
  23. Romagnani S, Del Prete G, Maggi E, et al. Human TH1 and TH2 subsets. Int Arch Allergy Immunol. 1992; 99:242-245. (Biology).
  24. Street NE, Mosmann TR. Functional diversity of T lymphocytes due to secretion of different cytokine patterns. FASEB J. 1991; 5:171-176. (Biology).
  25. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry.. J Immunol Methods. 1998; 212(1):89-98. (Biology). View Reference
View All (25) View Less
341117 Rev. 1

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