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Product Details
BD Horizon™
FCGR1; FcRI; Fc-gamma RI; IgG Fc Receptor I; High affinity IgG FcR1
Human
Mouse BALB/c IgG1, κ
Human FcγRI
5 µl/test
ASR
Preparation And Storage
The antibody reagent is stable until the expiration date shown on the label when stored at 2° to 8°C. Do not use after the expiration date. Do not freeze the reagent or expose it to direct light during storage or incubation with cells. Keep the reagent vial dry. Do not use the reagent if you observe any change in appearance. Precipitation or discoloration indicates instability or deterioration.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from BD Horizon™ BV421 may be observed. Therefore, we recommend that individual compensation controls be performed for every BD Horizon™ BV605 conjugate.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
666660 Rev. 1
Antibody Details
MD22
The MD22 monoclonal antibody specifically recognizes CD64 which is also known as Fc-gamma receptor I (FcgR1) or FcγRI. CD64 is a ~72 kDa type I transmembrane glycoprotein that is encoded by FCGR1A (Fc fragment of IgG receptor Ia). CD64 functions as a high affinity receptor for human IgG. The CD64 antigen is expressed on monocytes, macrophages, at low levels on polymorphonuclear neutrophils (PMNs), and on a subpopulation of circulating dendritic cells. The CD64 antigen is an early granulomonocytic lineage marker on CD34+ hematopoietic progenitors. Expression of the CD64 antigen increases transiently in cases of sepsis. The CD64 antigen functions in both innate and adaptive immune responses, and mediates endocytosis, phagocytosis, antigen presentation, antibody-dependent cellular cytotoxicity, cytokine release, and superoxide generation. The CD64 antigen associates with the signal-transducing γ-chain homodimer of Fc receptors to form the functional high affinity FcγRI complex. Ligation of the CD64 antigen leads to the activation of the protein tyrosine kinases, hck and lyn.
666660 Rev. 1
Format Details
BV605
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV605
407 nm
605 nm
666660 Rev.1
Citations & References
View product citations for antibody "666660" on CiteAb
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666660 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Analyte Specific Reagent. Analytical and performance characteristics are not established.
Documents are subject to revision without notice. Please verify you have the correct revision of the document, and always refer back to BD's eIFU website for the latest and most up to date information.