A Method to Study Tissue Microenvironments at Single-Cell Resolution
Spatial transcriptomics is a rapidly evolving field aiming to capture transcriptomic features and spatial relationships of cells within tissues.
A High-Throughput Sample Processing Pipeline with a 28-color Flow Cytometry Panel
The complexity of the immune system entails the simultaneous analysis of several parameters to gather meaningful insights. The advancements in flow cytometry instrumentation and the availability of reagents in recent years have facilitated high-dimensional analysis of immune cells to a tremendous extent.
A Novel Multiplex Flow Proxy Assay for the Simultaneous Measurement of Multiple Biomarkers
Combining the analysis of protein biomarkers to genome-wide expression profiling at the single-cell level provides deeper scientific insights than previously possible.
Antigen-Specific B Cells and Chronic Infection: The OMIP Panels
Antigen-specific B cells originating in the bone marrow prevent and control various infections through antibody production, professional antigen presentation and cytokine secretions.
New Strategy for Phenotyping Human Dendritic Cells and Monocyte Cells
Recent technological developments in single-cell analysis have significantly advanced our understanding of the subset heterogeneity of antigen-presenting cells. Mair et al. responded to the rising suggestions of an updated definition of human dendritic cells and monocyte cells based on functional and transcriptional signatures, as well as the expression of key transcription factors and surface markers.
Early Life Imprinting and Immune System Responses to Bifidobacteria Colonizations
There is mounting evidence to suggest that during the first three months of life the infant gut microbiome is crucial for immune development. Specialized microbes in the infant gut that rely on breast milk for nutrients play a key role in immune-microbe interactions
A New 16-Color Flow Cytometry Panel Protocol for the Functional Phenotyping of Circulating Human Cytotoxic
T Cells and NK Cells
Building a panel for the intracellular staining of immune cells could pose several challenges. A protocol published recently in Cell Press STAR Protocols takes these into account and provides a detailed workflow for efficiently phenotyping circulating human cytotoxic T cells and NK cells.
Single-Cell Genomic Approaches for Developing the Next Generation of Immunotherapies
In recent times, developments in genomics, proteomics and single-cell technologies, coupled with advances in allied areas like computing and antibody engineering, have enabled significant progress in drug development.
Using Flow Cytometry and Single-Cell Multiomics for Analyzing COVID-19 Immune Responses
The human immune system exerts coordinated responses capable of overcoming a broad spectrum of pathogenic challenges, including viral infections. Understanding the nature and sequence of these responses paves the way for targeting specific cells for further translational research.
High-Parameter Flow Cytometry for Deep Immune Profiling of COVID-19 Patients
The SARS-CoV-2 virus infection presents a range of clinical manifestations, ranging from mild or no symptoms to moderate to severe respiratory illnesses, such as ARDS (acute respiratory-distress syndrome) and multiorgan failure, to death. The innate immune defense mounted by the body involves pattern recognition receptors (PAR), which activate an array of transcription factors through a signal transduction pathway, ultimately resulting in the secretion of different interferons and chemokines.
Immunotyping of Natural Killer (NK) Cells in COVID-19 Patients Using High-Parameter Flow Cytometry
SARS-CoV-2 infection elicits a spectrum of immune responses, activating both the innate and adaptive immune systems. Whether these immune responses are targeted and controlled or misdirected and uncontrolled could determine the outcome of the disease. Recent studies using deep immunoprofiling have indicated that the nature of immune responses can also determine the severity of the disease.
