BD FACSuite™ Software

Taking productivity to the next level

A true evolution in flow cytometry is empowered by BD FACSuite™ software. It enables BD analyzers to provide standardization, consistency and flexibility while generating the highest quality reproducible data possible with the technology available today.

Setup Analysis Sample Data

Simplified Workflow

BD FACSuite software minimizes the number of user interactions with the instrument so that the user can spend more time working with samples. BD FACSuite software users can complete tasks at the click of a button for key workflows, from startup through instrument setup, sample acquisition, data analysis and system shutdown.

Streamlined Setup

BD FACSuite software streamlines instrument setup to achieve a new level of ease of use and reproducibility in cytometer setup and subsequent analysis.

BD FACSuite™ CS&T Research Beads are used to check the cytometer performance and automatically make adjustments, ensuring consistent values from day to day and experiment to experiment. Comprehensive checks during system initialization and setup, including performance QC, verify system performance, ensure that detector settings are optimal and triggers the automated laser alignment process if required. The entire setup process can be performed without manual intervention.

A New Approach Enabling Easy, Flexible Compensation

Accurate compensation is absolutely necessary for proper analysis of multicolor flow cytometric experiments, but it has always been a challenging and time-consuming process for users.

In the past, generating accurate compensation matrices typically required running individual compensation controls for every fluorochrome used at least once a day. Thus, for a five-color experiment, all five fluorochromes would be run initially to determine their spillover values (SOVs) for a compensation matrix. To subsequently add a sixth fluorochrome to the experiment, users would have to re-run all six at the same time.

BD FACSuite software minimizes the frequency and number of compensation controls required by taking advantage of the accurate, reproducible instrument setup provided by a combination of BD FACSuite™ CS&T Research Beads and BD FACSuite™ FC Beads. A single 20-minute procedure using BD FACSuite FC Beads determines SOVs that are gain-independent. These values are valid for 30 days. To add a new fluorochrome to the system, users run just one control for the new fluorochrome. Finally, the calculation of gain-independent SOVs lets users modify photomultiplier tube voltages if required without re-running compensation controls. All SOVs are automatically recalculated.

Together, these features mean time savings every day, since the BD FACSVerse™ system significantly reduces the frequency and complexity of the compensation process. It does this while increasing the accuracy and reproducibility of SOVs, and therefore the final experimental results.

Automatic Startup, System Idle, and Shutdown

To increase laboratory efficiency and ensure the system is ready by a specified time each day, a timer in the software can be set to turn on the instrument and complete initialization and warmup routines. BD FACSuite software can also be programmed to shut down the BD FACSVerse system at the end of the worklist run or when the system is idle for a specified period of time. In the case of idle time shutdown, a dialog communicates that the system will be shutting down, so that users can cancel the shutdown if desired.

BD FACSuite Home Page

Representative CTN Data Acquired on a BD FACSVerse System

BD FACSuite Setup and QC Workspace

Streamlined Approach to Application Support

BD FACSuite™ software provides optional BD pre-defined research assays for apoptosis, cell cycle, cell proliferation, and cytokine detection that are matched with BD reagent kits for reproducible results.

User-defined experiments created in BD FACSuite software can be converted to reusable assays that include relevant settings, acquisition and analysis worksheets, gating strategies, and report parameters to reduce variability of data between users and between laboratories for the same application.

In addition, before samples are acquired, the software verifies that the system’s current configuration matches the configuration needed to run the experiment. If it identifies a mismatch, such as a different optical filter-mirror unit, the system alerts the user. This check can help streamline workflow and reliability of results.

Intuitive Workspaces for Ease of Adoption

The user interface is designed around three common workflows: setup, acquisition, and analysis. Workspaces provide users the functionality they need to perform the tasks relevant to a given workflow. For example, in the Setup workspace, users find the QC tools and tools for instrument configuration. In the Experiment workspace, users can adjust tube settings, plots and gates. These workflow-based workspaces reduce clutter to help users complete their tasks more quickly. Administrative functions such as user management, diagnostics and periodic maintenance procedures are accessible only to authorized personnel.

Multitasking Support for Added Productivity

The functions in BD FACSuite software workspaces operate both together and independently. The software supports multitasking, enabling users to work on several tasks simultaneously, such as performing data analysis while the system is acquiring data. A user even can prioritize a single sample for immediate analysis. Multitasking is yet another way that the software promotes a more flexible, productive workflow.

BD FACSuite Library Workspace

BD FACSuite Assay Worklist Workspace

BD FACSuite Experiment Workspace

Reliable Data Across Sites

For the first time, researchers have control to ensure consistent instrument setup and consistent experiment results (acquisition, analysis, gating strategies, and report parameters) in a single application—BD FACSuite software. Free from concerns about instrument and human variables, scientists can move forward in their research, reliably reproducing an experiment from one instrument to another and across sites.

Support for Security Standards and More

BD FACSuite software supports 21 CFR Part 11 guidelines for Electronic Records and Signatures to protect data integrity. This includes unique user identification and passwords, electronic signatures, and audit trails. In addition to meeting the requirements specified in the 21 CFR Part 11 guidelines, BD FACSuite software encrypts files and uses specially designed algorithms to ensure that data is not manipulated by unauthorized entities.

Optional Remote Systems Management

Optional remote systems management software enables BD field service personnel to access a networked BD FACSVerse™ system and review system performance and status using the System Health Report. This allows the BD service team to determine the nature and extent of the issue, and if possible repair the issue remotely to ensure minimal downtime. It also helps the BD service team to be better prepared if on-site support is required.

Greater Speed and Freedom for Data Analysis

BD FACSuite software offers a variety of ways to visualize data and tools to easily look more closely at data for analysis.

The data analysis tools let users examine data as dot plots, histograms, contours and density plots. For a better look at data, new hybrid contour plots combine standard dot plots with the coloring of contour plots. The information about contour statistics is available by hovering over the plot. A new capability lets users overlay dot plots within the same or different files. Gating tools include ellipses, polygons and free-hand polygons, as well as the traditional rectangle. Gates can be rotated as needed, and with a zoom feature, users can zoom in or out of areas of interest.

Several types of new quadrant markers provide additional flexibility, including a hinged marker to more precisely identify quadrants of interest.

An expression editor is also built into BD FACSuite software to speed analysis. The expression editor lets users develop mathematical expressions and assign them to keywords. Users can hover over a result for a display of the formula that was used to calculate it.

To assist with presentation, vector graphics provide superior quality images that can be easily cut and pasted into third-party software such as Microsoft® PowerPoint®. A new report generator produces high-quality reports that include color graphics and can be easily customized by dragging and dropping report elements.

Greater Speed and Freedom for Data Analysis

Representative CTN Data Acquired on a BD FACSVerse System

Greater Speed and Freedom for Data Analysis

Making global collaboration possible

BD FACSuite™ software provides an easy-to-use yet flexible interface for data acquisition and analysis with the BD FACSLyric™ system. The application is designed for user-defined assays and collaboration through the Assay Portability feature, and provides a simple, reproducible and transferrable setup. Instrument QC and setup are easy and fast.

    

   

Streamlined

BD™ CS&T Beads are used to check the cytometer performance and automatically make adjustments, ensuring consistent values from day to day and experiment to experiment. Comprehensive checks during system initialization and setup, including performance QC, verify system performance, ensure that detector settings are optimal and trigger the automated laser alignment process if required. The entire setup process can be performed without manual intervention.

Universal

Universal Setup ensures that setup is an easy, quick procedure for Instrument QC and Assay Setup for user-defined assays.

Time Saving

Measuring spillover in no longer a daily or weekly activity. On the BD FACSLyric flow cytometer, spillover value measurement is performed with pre-dispensed dried beads and reduced to once every 60 days. Spillover values are maintained within the system and updated automatically with PMTV changes.

Reproducible and Portable

A key aspect of effectively using multicolor flow is ensuring that the experiment can be performed consistently over time and across instruments. Universal setup ensures consistency of results over time. The Assay Portability feature within BD FACSuite software enables consistency across multiple systems, ensuring reproducibility over time, across operators and across instruments.

Reproducible Across Instruments

To help enable reproducible results day to day, between instruments, or between sites, it is critical that cytometer setup is standardized and efficient. The BD FACSLyric system enables users to accurately adjust gains to achieve fluorescence (median fluorescence intensity, MFI) target values that are consistent across the instrument. Setup standardization between instruments or across sites helps enable equivalency between assays or experiments. When assay settings are imported across six BD FACSLyric cytometers, target MFI values show high reproducibility and a variance below 10%.

  Median Fluorescence Intensity
Instrument
Antigen Fluorochrome 1 2 3 4 5 6 %CV
CD4 FITC 1,891 1,699 1,816 1,865 1,661 1,691 5.6%
CD4 PE 16,781 16,781 17,393 18,363 16,993 17,482 3.5%
CD4 PerCP-Cy™5.5 4,615 4,847 4,546 5,012 4,661 4,913 3.9%
CD4 PE-Cy™7 25,402 27,600 26,066 29,213 30,510 26,393 7.2%
CD4 APC 20,064 21,163 20,768 20,600 20,990 18,848 4.2%
CD4 APC-R700 25,238 27,083 26,202 27,982 29,449 26,147 5.6%
CD4 APC-H7 7,406 7,662 7,607 8,000 8,119 7,634 5.6%
CD4 V450 5,234 5,220 5,473 5,299 5,631 5,581 3.3%
CD4 V500-C 2,294 2,332 2,338 2,354 2,318 2,039 5.2%

Between-instrument Reproducibility of Target MFI Values

Daily QC using one lot of BD CS&T beads was run on six 10-color BD FACSLyric cytometers. Target MFI values were defined and single-color stains of peripheral blood samples acquired on BD FACSLyric Instrument #1. Settings from Instrument #1 were imported onto, and the same set of stains acquired on, Instruments 2 through 6. The MFI of the relevant positive populations was measured for all parameters across all instruments. The %CV is shown.

Resources Bead Lots Files

BD FACSLyric™ Bead Lot Files

Name Bead Lot Files Status Part Number
BD™ CS&T

When running a BD FACSLyric™ 12-color IVD instrument, we recommend using lot 9051880 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

910898_9273345
910898_9228017
910898_9163529
910898_9141729
910898_9093951
910898_9063854
910898_9051880
910898_8164664
910898_7298995
910898_7235842
910898_7151720
910898_7081518
IVD 662413 (50 Tests)
662414 (150 Tests)
BD™ FC Beads 7-Color Kit

When running a BD FACSLyric™ 12-color IVD instrument, we recommend using lot 8312586 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

662961_9262875
662961_9197331
662961_9193130
662961_9036945
662961_8312586
662961_8156974
662961_8057900
662961_8024612
662961_8008815
662961_7250992
IVD 662961
BD™ CS&T RUO

When running a BD FACSLyric™ 12-color instrument, do not use BD™ CS&T RUO bead lots 911013_7073501 or 911013_7122820. Using these bead lots will result in an incorrect setup of the BV711 and BV786 channels. All other BD CS&T RUO bead lots can be used with the BD FACSLyric 12-color RUO instrument.

911013_9177921
911013_9218757
911013_9122813
911013_8299836
911013_8207638
911013_7298994
911013_7235845
911013_7191562
911013_7122820
911013_7073501
RUO 661414 (50 Tests)
661415 (150 Tests)
BD™ FC Bead FITC

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334508 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661615_9206313
661615_9158718
661615_9136628
661615_9105626
661615_9036861
661615_8334508
661615_8270941
661615_8201669
661615_8179856
661615_8030503
661615_7297736
661615_7251544
661615_7171992
661615_7156984
661615_7150892
661615_7115722
RUO 661615
BD™ FC Bead PE

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334509 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661616_9158729
661616_9123737
661616_9100715
661616_9060565
661616_9035690
661616_8334509
661616_8270947
661616_8179694
661616_8158880
661616_8012796
661616_7278887
661616_7251545
661616_7171598
661616_7152793
661616_7150916
661616_7115724
RUO 661616
BD™ FC Bead APC

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8325992 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661620_9270470
661620_9206335
661620_9140548
661620_9098598
661620_9021735
661620_8325992
661620_8215969
661620_8150554
661620_8115652
661620_8099709
661620_7297740
661620_7254524
661620_7171602
661620_7150934
661620_7115725
RUO 661620
BD™ FC Bead V450

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8317652 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661623_9240707
661623_9186906
661623_9142930
661623_9120771
661623_9063843
661623_9017540
661623_8317652
661623_8240874
661623_8215964
661623_8186566
661623_8073509
661623_8012739
661623_7195605
661623_7171606
661623_7150941
661623_7115727
RUO 661623
BD™ FC Bead PerCP-Cy™5.5

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334522 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661619_9240677
661619_9151785
661619_9120718
661619_9060570
661619_8334522
661619_8296643
661619_8150999
661619_8150997
661619_8023538
661619_7311625
661619_7292967
661619_7171607
661619_7150699
661619_7115729
RUO 661619
BD™ FC Bead APC-H7

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8320661 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661621_9240691
661621_9158714
661621_9140559
661621_9105633
661621_9036867
661621_9036857
661621_9003755
661621_8320661
661621_8240994
661621_8158885
661621_8149649
661621_8073508
661621_7311653
661621_7195603
661621_7171608
661621_7150694
661621_7115732
RUO 661621
BD™ FC Bead PE-Cy™7

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8325994 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661617_9240672
661617_9140537
661617_9092601
661617_9036863
661617_8325994
661617_8215967
661617_8150547
661617_7348658
661617_7171609
661617_7150921
661617_7150698
661617_7150697
661617_7115737
RUO 661617
BD™ FC Bead V500-C

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334550 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661624_9228007
661624_9206325
661624_9158721
661624_9142932
661624_9120753
661624_9036859
661624_8334550
661624_8323641
661624_8297864
661624_8270978
661624_8206696
661624_8158878
661624_8073516
661624_7311627
661624_7195609
661624_7171610
661624_7150944
661624_7115984
RUO 661624
BD™ FC Bead APC-Cy™7

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8325996 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661622_9206337
661622_9142931
661622_9105629
661622_9030851
661622_8325996
661622_8215965
661622_8179849
661622_8150507
661622_7348545
661622_7195604
661622_7171596
661622_7194807
661622_7150692
661622_7115991
RUO 661622
BD™ FC Bead PerCP

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334506 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661618_9206332
661618_9140515
661618_9123739
661618_9091554
661618_8334506
661618_8297859
661618_8206694
661618_8149627
661618_8012731
661618_7251540
661618_7171593
661618_7150701
661618_7115992
RUO 661618
BD™ FC Bead BV605

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334562 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661626_9176744
661626_9137789
661626_9120765
661626_9036869
661626_8334562
661626_8296646
661626_8240997
661626_8221609
661626_8213538
661626_8149631
661626_8073520
661626_8022798
661626_7311624
661626_7255586
661626_7213775
661626_7195610
661626_7115746
RUO 661626
BD™ FC Bead APC-R700

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334514 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661625_9206315
661625_9158794
661625_9140534
661625_9123746
661625_8334514
661625_8241934
661625_8226913
661625_8141509
661625_8073504
661625_8023537
661625_7311656
661625_7220936
661625_7194809
661625_7130936
661625_7130932
661625_7115745
RUO 661625
BD™ FC Bead BV421

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334528 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661627_9158726
661627_9142933
661627_9060583
661627_8334528
661627_8270988
661627_8150555
661627_8057545
661627_7299724
661627_7177726
661627_7138589
RUO 661627
BD™ FC Bead BV510

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8325997 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661628_9199470
661628_9158772
661628_9123742
661628_9028802
661628_8325997
661628_8257591
661628_8198724
661628_8074638
661628_7299725
661628_7177754
661628_7138591
RUO 661628
BD™ FC Bead BV711 661629_9252129
661629_9141706
661629_8215962
661629_8205891
661629_8172974
661629_8086588
661629_7276594
RUO 661629
BD™ FC Bead BV786 661630_9242607
661630_9176776
661630_9142929
661630_9060582
661630_9035662
661630_8213535
661630_8191881
661630_8172976
661630_8086613
661630_7276595
RUO 661630
BD™ FC Bead BB515

When running a BD FACSLyric™ 12-color instrument, we recommend using lot 8334511 or newer. This new lot of beads, and all future lots, have been optimized for the 12-color instrument.

661631_9176781
661631_9120768
661631_8334511
661631_8264818
661631_8115648
661631_7278888
661631_7180619
661631_7138592
RUO 661631


For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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