BD FACSDiva™ Software

Expanding confidence and flexibility in today’s integrated laboratory

BD FACSDivaTM software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for today's busy laboratory.

BD FACSDiva software provides new features to help users integrate flow systems into new application areas, including index sorting for stem cell and single-cell applications, as well as automation protocols for high-throughput and robotic laboratories.

Overview Setup Tracking Organization

Robust features from setup through analysis

BD FACSDiva™ software provides one of the most complete and robust feature sets available for flow cytometry.

BD FACSDiva v8.0.3 Software

BD FACSDiva v8.0.3 software continues to provide powerful index sorting, along with more routine software operation features including overlays, undo/redo, and multiple copying and pasting of all worksheet elements. This version runs on the Windows® 7 32-bit operating system. A complete list of BD FACSDiva software features is listed in the following specification table.

The index sorting feature enables laboratories to sort cells into single wells of plates, and also easily identify them on plots, in statistics views, and in a detailed results file.

Index sorting

Feature Specification
Cytometers Supported v8.0.3: BD FACSCanto™, BD FACSCanto™ II, BD FACSAria™ II, BD FACSAria™ III, BD FACSAria™ Fusion, BD™ LSR II, BD LSRFortessa™, and BD LSRFortessa™ X-20
Options Supported BD FACS™ Loader for the BD FACSCanto platform, BD™ High Throughput Sampler for the BD FACSCanto and BD LSR platforms, and BD FACS™ Accudrop for the BD FACSAria platform
Plates Supported U, V, and flat-bottom 96-well plates, flat-bottom 384-well plates, Terasaki plates (BD FACSAria only)
Laser/Filter Support 145 (See Table 1 in Qr and Br in BD FACSDiva™ Software: Parameters for Characterizing Detector Performance)
Event Display User-Defined Event Number, User-Defined Percentage
Standard Gating Interval, Rectangle, Polygon, Quadrants, Hinged Quadrants
Logical Gating And, Or, Not, and Rest Of
Auto Gating Auto-Polygon, Auto-Interval, Snap-To Polygon, Snap-To Interval
Standard Statistics Minimum, Maximum, Geometric Mean, Mean, Median, Standard Deviation, Percent Coefficient of Variation, Mode
Robust Statistics Robust Standard Deviation, Robust Percent Coefficient of Variation
Analysis Types Manual or Batch
Plot Types Histogram, 2D Dot Plot, 2D Contour Plot, 2D Density Plot
Plot Alignment Tools Left, Right, Bottom, Top, Distribute Horizontally, Distribute Vertically
Performance Tracking Measurements PMTV, Qr, Br, Bright Bead Target Value, Bright Bead %rCV, Mid Bead Median, Mid Bead %rCV, Dim Bead Median, Dim Bead %rCV, Forward Scatter Laser Delay, Fluorescence Laser Delay, Laser Current, Laser Power, Fluidics Pressure
Scaling Linear, Log, Biexponential
Automated Setup Define Baseline, Check Performance, Auto Compensation, Reset Target Values
Export Formats JPG, TIFF, PNG, BMP, XML, CSV, PDF
Export Files Experiments, FCS Files, Statistics, Templates
Levey-Jennings Plots Up to 30
Operating System v8.0.3: Microsoft® Windows 7 Professional, Service Pack 1 (32-bit)

Built-in expertise for consistent, reliable results

BD FACSDiva™ software supports data integrity and helps ensure reproducible results by enabling automated cytometer setup.

Experiment and Application Setup

BD FACSDiva software provides convenient and easy experiment layout tools to simplify creation of experiments. These tools provide a graphical view of the entire experiment and allow the quick addition of labels, keywords, and acquisition attributes to a single tube, multiple tubes, specimens, or the entire experiment. In addition, BD FACSDiva software provides the ability to store experiment, analysis, and panel templates for re-use without sacrificing data integrity.

With the BD FACSDiva application settings feature, users can save their specific settings to properly resolve their populations of interest. Once a daily performance check is performed to update settings, application settings can be applied to ensure that the particular applications with these settings are running consistently from day to day.

Automated Daily Setup and QC

A single daily setup run using BD FACSDiva software and BD FACSDiva™ CS&T research beads* supports consistency and accuracy of data acquisition. Key cytometer setup values for PMT voltages, laser delay, and area scaling factors are automatically calculated and adjusted to maintain optimal performance levels over time. This automation reduces startup time to approximately five minutes and eliminates multiple error-prone and expensive data acquisitions and calculations.

Note that because of a change in the beads for BD FACSDiva software v7.0 and v8.0, upgrading from BD FACSDiva software v6.x to v8.0 will cause a loss of existing baselines and application settings. All baselines, performance checks, and application settings will have to be recreated, as needed. See the BD FACSDiva Software Reference Manual and the BD Cytometer Setup and Tracking Application Guide for more details.

Cytometer Configuration Setup

BD FACSDiva software provides a graphic of the cytometer's physical optical configuration, showing the layout of detectors, filters, and mirrors. This allows users to select the correct configuration for their work. Administrators can create specialized configurations for particular applications by dragging filters, mirrors, and fluorochromes onto a representation of the optical bench.

Automatic cytometer adjustments

Experiment Layout

Visual tools for cytometer configuration setup

Automated performance tracking simplifies quality control

BD FACSDiva™ software automatically tracks a wide range of performance metrics for each detector and laser in the system and displays the results in Levey-Jennings charts. Alarm criteria can also be set to automatically notify users if performance metrics fall outside these boundaries.

Download Bead Lot Files

Levey-Jennings charts

Automatically generated performance reports

Proactive Performance Monitoring

On a daily basis, BD FACSDiva software automates tracking of all performance metrics, including detection efficiency, standard deviation of electronic noise, laser current, and optical background. Viewing these metrics in interactive Levey-Jennings charts, administrators can visually adjust displays and time scales. The software allows notification when performance metrics fall outside specified boundaries, for early troubleshooting and correction.

Efficient and accurate experiment setup

BD FACSDiva™ software helps users manage experimental details with easy-to-use interfaces and controls.


BD FACSDiva software simplifies data acquisition with a dashboard that provides the tools for immediately viewing acquisition status and for setting acquisition stopping criteria. The application settings feature automatically adjusts voltage settings to daily changes in cytometer performance, so that users can run existing applications more simply and quickly.


BD FACSDiva software provides a wide array of basic analysis features, enabling multiple applications to run on the BD FACSCanto™, BD™ LSR, and BD FACSAria™ cytometers. These include gating tools to automatically find a population, biexponential scaling to completely see populations that might have negative relative fluorescence intensities, and even overlays to compare multiple tubes or samples.


Worksheets are configurable using pre-defined and customizable headers and footers. Snap-to grids allow for easy alignments of plots, comments, and statistics. Previewing and PDF creation can be completed within the BD FACSDiva interface.

Global worksheet for data reporting


Data management

BD FACSDiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. Along with built-in database and database utilities, BD FACSDiva software provides automated data export in a variety of formats including XML, FCS, and even ZIP, for multiple files concurrently. Manual export of FCS files creates an experiment folder structure in the operating system for easy identification of data files without the need to open them.

Single-tube setup for common laser and filter combinations

Setting PMT voltages

Example sections of a daily performance report

BD Phosflow monocyte/NK cell activation

Eight-color immunophenotyping on BD FACSCanto II cytometer

Resources Bead Lot Files

Application Notes

Product Information Sheets

Quick Reference Guides

Technical Bulletins



User Guide


Installation Instructions

Name Bead Lot Files Status Part Number
BD FACSDiva™ CS&T Bead Lot Files (for BD FACSDiva Version 7 or later) RUO 910858
BD FACSDiva™ CS&T Bead Lot Files (for BD FACSDiva Version 7 or later) IVD 910859
BD™ Cytometer Setup and Tracking (CS&T) Bead Lot Files (for BD FACSDiva Version 6) RUO 910723

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Microsoft and Excel are registered trademarks of Microsoft Corporation.

Microsoft and Windows are registered trademarks of Microsoft Corporation.

*The BD Cytometer Setup and Tracking application and BD FACSDiva CS&T research beads are For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Brilliant Violet is a trademark of Sirigen Group Ltd.

CF is a trademark of Biotium, Inc.

This site uses cookies. If you click accept cookies then all cookies will be written. Please review our cookies policy and configure your cookies for your experience.