BD Phosflow™ T Cell Activation Kit
(RUO)
Preparation And Storage
Store the fluorescent antibodies, BD CompBeads, Stain Buffer, Treated and Untreated Control Cells, and Lyse/Fix Buffer at 2 to 8°C. Do not freeze. The fluorescent antibodies and BD CompBeads should be protected from prolonged exposure to light. Store the Perm Buffer III at –20°C.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Development References (4)
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Montag DT, Lotze MT. Rapid flow cytometric measurement of cytokine-induced phosphorylation pathways [CIPP] in human peripheral blood leukocytes. Clin Immunol. 2006; 121(2):215-226. (Biology). View Reference
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Montag DT, Lotze MT. Successful simultaneous measurement of cell membrane and cytokine induced phosphorylation pathways [CIPP] in human peripheral blood mononuclear cells. J Immunol Methods. 2006; 313(1-2):48-60. (Biology). View Reference
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Perez OD, Mitchell D, Campos R, Gao GJ, Li L, Nolan GP. Multiparameter analysis of intracellular phosphoepitopes in immunophenotyped cell populations by flow cytometry. Curr Protoc Cytom. 2005; 6.20.1-6.20.22. (Biology). View Reference
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Perez OD, Mitchell D, Jager GC, et al. Leukocyte functional antigen 1 lowers T cell activation thresholds and signaling through cytohesin-1 and Jun-activating binding protein 1. Nat Immunol. 2003; 4(11):1083-1092. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
