Description
CD2, clone L303.1, and CD2R, clone L304.1, are each derived by the fusion of SP2/0 myeloma cells with splenocytes from BALB/c mice immunized with CD3-activated human T lymphocytes. The CD2 molecule is an Mr 50-kilodalton (kd) human lymphocyte antigen. At least three different functional epitopes of CD2 have been defined. One epitope (recognized by Leu™-5b) is the sheep red blood cell (SRBC) receptor. The other two epitopes, CD2 and CD2R, are binding sites for monoclonal antibodies that, when used together, deliver signals for polyclonal T-lymphocyte activation.
Preparation And Storage
The monoclonal antibody is supplied as 125 μg each of CD2 and CD2R in 250 μL of phosphate-buffered saline (PBS) containing 0.01% sodium azide. The vial should be stored at 2° to 8°C. Each reagent is stable for the period shown on the bottle label when stored as directed.
| Description | Clone | Isotype | EntrezGene ID |
|---|---|---|---|
| CD2 Purified | L303.1 | IgG2a, κ | 3689 |
| CD2R Purified | L304.1 | IgG1, κ | 914 |
Development References (6)
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Huet S, Wakasugi H, Sterkers G, et al. T cell activation via CD2 (T, gp50): The role of accessory cells in activating resting T cells via CD2. J Immunol. 1986; 137:1420-1428. (Biology).
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Lanier LL, Phillips JH, Hackett J Jr, Tutt M, Kumar V. Natural killer cells: definition of a cell type rather than a function. J Immunol. 1986; 137(9):2735-2739. (Biology). View Reference
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Maino VC, Suni MA, Ruitenberg JJ. Rapid flow cytometric method for measuring lymphocyte subset activation.. Cytometry. 1995; 20(2):127-33. (Biology). View Reference
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Meuer SC, Hussey RE, Fabbi M, et al. An alternative pathway of T cell activation: A functional role for the 50 kD T11 sheep erythrocyte receptor protein. Cell. 1984; 36:897. (Biology).
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Meuer SC. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:270-272.
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Parish CR, McPhun V, Warren HS. Is a natural ligand of the T lymphocyte CD2 molecule a sulfated carbohydrate?. J Immunol. 1988; 3498-3504. (Biology).
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