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BD Horizon™ RY610 Mouse Anti-Human TCR Cβ1
Clone JOVI.1 (RUO)
Two-color flow cytometric analysis of TCR Cβ1 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD3 (Cat. No. 562426; Left Plots), Alexa Fluor™ 488 Mouse Anti-Human CD4 (Cat. No, 557695; Center Plots), or BD Horizon™ BUV395 Mouse Anti-Human CD8 (Cat. No. 563795; Right Plots) antibodies and with either BD Horizon™ RY610 Mouse IgG2a, κ Isotype Control (Cat. No. 571157; Upper Plots) or BD Horizon™ RY610 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 571263/571323; Lower Plots) as indicated. The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plots showing the correlated expression of TCR Cβ1 (or Ig Isotype control staining) versus CD3, CD4, or CD8 were derived from gated events with the forward and side light-scatter characteristics of intact Human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Two-color flow cytometric analysis of TCR Cβ1 expression on Human peripheral blood T lymphocytes. Human peripheral blood cells were stained with BD Horizon™ BV421 Mouse Anti-Human CD3 (Cat. No. 562426; Left Plots), Alexa Fluor™ 488 Mouse Anti-Human CD4 (Cat. No, 557695; Center Plots), or BD Horizon™ BUV395 Mouse Anti-Human CD8 (Cat. No. 563795; Right Plots) antibodies and with either BD Horizon™ RY610 Mouse IgG2a, κ Isotype Control (Cat. No. 571157; Upper Plots) or BD Horizon™ RY610 Mouse Anti-Human TCR Cβ1 antibody (Cat. No. 571263/571323; Lower Plots) as indicated. The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plots showing the correlated expression of TCR Cβ1 (or Ig Isotype control staining) versus CD3, CD4, or CD8 were derived from gated events with the forward and side light-scatter characteristics of intact Human lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ Software.
Product Details
BD Horizon™
T-cell receptor beta locus; TCRB; TRB; TRB@; TRCB1
Human (QC Testing)
Mouse IgG2a, κ
Lymphoid cells from Mouse transgenic for Human HA1.7 cell TCRbeta
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_3686367
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- An isotype control should be used at the same concentration as the antibody of interest.
- CF™ is a trademark of Biotium, Inc.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- For U.S. patents that may apply, see bd.com/patents.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
Companion Products
Stain Buffer (FBS) RUO
Size
500 ML
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 ML
Cat No.
554657
RY610 Mouse IgG2a, κ Isotype Control RUO
Size
0.1 mg
Cat No.
571157
Lysing Buffer RUO
Size
100 ML
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 ML
Cat No.
349202
BV421 Mouse Anti-Human CD3 RUO
Size
100 Tests
Cat No.
562426
571263 Rev. 1
Antibody Details
JOVI.1
The JOVI.1 monoclonal antibody recognizes an epitope common to a large proportion of human CD4+ or CD8+ T lymphocytes that express the T cell receptor beta chain (TCRβ). The antibody was generated from a mouse immunized with transgenic mouse lymphoid cells that expressed the rearranged human Vβ3-Cβ1 TCR chain derived from the cloned human HA1.7 T helper cell. This antibody reacts with TCR-Cβ1+ T cells and one of several different TCRβ V regions, but not with TCR-Cβ2+ T cells. JOV1.1 antibody reportedly recognized several Cβ1 TCR expressing cell lines or clones including Jurkat, CH7C17, and HA1.7 cells. The JOVI.1 antibody can be used to stimulate proliferative responses by JOVI.1-positive T cells. It can also reportedly be used for immunoprecipitation and to stain JOVI.1+ T cells in frozen tissue sections.
571263 Rev. 1
Format Details
RY610
The BD Horizon RealYellow™ 610 (RY610) Dye is part of the BD® family of yellow-green dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 557-nm and an emission maximum (Em Max) at 610-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RY610 can be used on both spectral and conventional cytometers and is designed to be excited by the Yellow-Green laser (561-nm) with minimal excitation by the 488-nm blue laser. For conventional instruments equipped with a Yellow-Green laser (561-nm), RY610 can be used as an alternative to PE-CF594 and we recommend using an optical filter centered near 610-nm (eg, a 610/20-nm bandpass filter). For spectral instruments equipped with a Yellow-Green laser (561-nm), it can be used in conjunction with PE-CF594.
RY610
557 nm
610 nm
571263 Rev.1
Citations & References
View product citations for antibody "571263" on CiteAb
Development References (3)
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Gil D, Schamel WWA, Montoya M, Sanchez-Madrid F, and Alarcon B. Recruitment of Nck by CD3-epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell. 2002; 109(7):901-912. (Clone-specific: Activation, Functional assay, Immunoprecipitation). View Reference
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San José E, Alarcón B. Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway.. J Biol Chem. 1999; 274(47):33740-6. (Clone-specific: Immunoprecipitation). View Reference
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Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ. Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992; 11(6):701-713. (Immunogen: Activation, Bioassay, Flow cytometry, Functional assay, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Stimulation). View Reference
571263 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.