Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human Myeloperoxidase antibody (Cat. No. 757761; Right Plot) in Human peripheral blood previously treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723), with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human Myeloperoxidase antibody (Cat. No. 757761; Right Plot) in Human peripheral blood previously treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723), with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human Myeloperoxidase antibody (Cat. No. 757761; Right Plot) in Human peripheral blood previously treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723), with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Multiparameter flow cytometric analysis using BD OptiBuild™ RB744 Mouse Anti-Human Myeloperoxidase antibody (Cat. No. 757761; Right Plot) in Human peripheral blood previously treated with BD Phosflow™ Lyse/Fix Buffer (Cat. No. 558049) to lyse erythrocytes and fix leukocytes followed by permeabilization with BD Perm/Wash™ Buffer (Cat. No. 554723), with corresponding IgG Isotype Control (Cat. No. 570519; Left Plot). Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer.
Product Details
BD OptiBuild™
MPO
Human (Tested in Development)
Mouse IgG1, κ
Purified Human Myeloperoxidase
Flow cytometry (Qualified)
0.2 mg/ml
4353
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
- The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
- Tandem fluorochromes contain both an energy donor and an energy acceptor. Although every effort is made to minimize the lot-to-lot variation in the efficiency of the fluorochrome energy transfer, differences in the residual emission from the donor may be observed. Additionally, multi-laser cytometers may directly excite both the donor and acceptor fluorochromes. Therefore, we recommend for every tandem conjugate, a matched individual single-stain control be acquired for generating a compensation or spectral unmixing matrix.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- For U.S. patents that may apply, see bd.com/patents.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Human BD Fc Block™ RUO
Size
0.25 mg
Cat No.
564220
RB744 Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
570519
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
Lysing Solution 10X Concentrate IVD
Size
100 mL
Cat No.
349202
757761 Rev. 2
Antibody Details
1B10
Reacts with myeloperoxidase (MPO), a hemoprotein of 140 kDa, composed of two heavy subunits of 52 kDa and two light chains of 15 kDa. MPO is stored in primary azurophilic granules of neutrophils and plays a major role in the bactericidal activity of neutrophils during phagocytosis. It catalyzes the generation of hypochlorous acid, a powerful oxidant. 1B10 antibody detects MPO in neutrophils, monocytes and HL-60 cells. This reagent is suitable for immunohistochemical staining of acetone-fixed, frozen tissue section, or formalin-fixed, paraffin-embedded tissue sections with TUF pretreatment, or zinc-fixed, paraffin-embedded tissue sections with no pretreatment.
757761 Rev. 2
Format Details
RB744
The BD Horizon RealBlue™ 744 (RB744) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 746-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB744 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), we recommend using an optical filter centered near 750-nm (e.g., a 750/60-nm bandpass filter).
RB744
Blue 488 nm
498 nm
746 nm
757761 Rev.2
Citations & References
View product citations for antibody "757761" on CiteAb
Development References (3)
-
Audrain MA, Baranger TA, Moguilevski N, et al. Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies. Clin Exp Immunol. 1997; 107(1):127-134. (Immunogen: ELISA, Western blot). View Reference
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Kennedy AD, Willment JA, Dorward DW, Williams DL, Brown GD, DeLeo FR. Dectin-1 promotes fungicidal activity of human neutrophils.. Eur J Immunol. 2007; 37(2):467-78. (Clone-specific: Western blot). View Reference
-
Lanza F, Latorraca A, Moretti S, Castagnari B, Ferrari L, Castoldi G. Comparative analysis of different permeabilization methods for the flow cytometry measurement of cytoplasmic myeloperoxidase and lysozyme in normal and leukemic cells. Cytometry. 1997; 30(3):134-144. (Biology). View Reference
757761 Rev. 2
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.