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      Purified Mouse anti-Human TRA-1-85 Antigen
      Purified Mouse anti-Human TRA-1-85 Antigen
      Flow cytometric analysis of TRA-1-85 expression on human cervical carcinoma (HeLa cell line). HeLa cells (ATCC® CCL 2™) were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal  antibody (solid line histogram) or with Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line histogram). The second-step reagent was FITC goat anti-Mouse Ig (Cat. No. 554001). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of the HeLa cell line. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.
      Purified Mouse anti-Human TRA-1-85 Antigen

      Immunoflourescent staining of Tra-1-85 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 47 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with Hoechst (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

      Purified Mouse anti-Human TRA-1-85 Antigen Purified Mouse anti-Human TRA-1-85 Antigen
      Flow cytometric analysis of TRA-1-85 expression on human cervical carcinoma (HeLa cell line). HeLa cells (ATCC® CCL 2™) were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal  antibody (solid line histogram) or with Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121; dashed line histogram). The second-step reagent was FITC goat anti-Mouse Ig (Cat. No. 554001). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of the HeLa cell line. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System.

      Immunoflourescent staining of Tra-1-85 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 47 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with Hoechst (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

      Product Details
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      BD Pharmingen™
      OKa, CD147, EMMPRIN, Basigin
      Human (QC Testing), Mouse (Lack of Reactivity Confirmed in Development)
      Mouse IgG1, κ
      Human Embryonal Carcinoma Cell Line
      Flow cytometry (Routinely Tested), Bioimaging, Immunofluorescence, Western blot (Tested During Development), Immunohistochemistry-frozen (Reported)
      0.5 mg/ml
      AB_2737956
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. An isotype control should be used at the same concentration as the antibody of interest.
      3. mTESR™1 is a trademark of StemCell Technologies.
      4. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
      5. All other brands are trademarks of their respective owners.
      6. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      7. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      563020 Rev. 1
      Antibody Details
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      TRA-1-85

      The TRA-1-85 monoclonal antibody (mAb) specifically binds to the high frequency blood group antigen OKa. The OKa blood group antigen is a 35-69 kDa glycoprotein also known as CD147, EMMPRIN, M6, or Basigin. The OKa group is widely expressed on most human tissues including, but not limited to kidney, peripheral blood leukocytes, liver, pancreas, colon, skin, and brain. TRA-1-85 mAb is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies. During development, the purified TRA-1-85 mAb was found to detect OKa blood group antigen by western blot analysis of cellular lysates, by immunofluorescent staining of fixed cells, and by flow cytometric analysis of live cells.

      563020 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      563020 Rev.1
      Citations & References
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      Development References (5)

      1. Choi KD, Yu J, Smuga-Otto K, et al. Hematopoietic and endothelial differentiation of human induced pluripotent stem cells. Stem Cells. 2009; 27(3):559-567. (Clone-specific: Flow cytometry). View Reference
      2. Draper JS, Pigott C, Thomson JA, Andrews PW. Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J Anat. 2002; 200:249-258. (Clone-specific: Flow cytometry). View Reference
      3. Olivier EN, Rybicki AC, Bouhassira EE. Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells. Stem Cells. 2006; 24(8):1914-1922. (Biology). View Reference
      4. Spring FA, Holmes CH, Simpson KL, et.al. The Oka blood group antigen is a marker for the M6 leukocyte activation antigen, the human homolog of OX-47 antigen, basigin and neurothelin, an immunoglobulin superfamily molecule that is widely expressed in human cells and tissues. Eur J Immunol. 1997; 27(4):891-897. (Clone-specific: Flow cytometry, Immunohistochemistry, Western blot). View Reference
      5. Williams BP, Daniels GL, Pym B, et al. Biochemical and genetic analysis of the OKa blood group antigen. Immunogenetics. 1988; 27:322-329. (Clone-specific: Western blot). View Reference
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      563020 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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