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Purified Mouse Anti-Human CD62L
Product Details
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BD Pharmingen™
L-selectin; LECAM-1; SELL; LNHR; LSEL; LAM-1; LEU8; PLNHR; TQ-1; MEL-14
Human (QC Testing)
Mouse BALB/c IgG2a, κ
Human Peripheral Blood T Lymphocytes
Flow cytometry (Routinely Tested), Functional assay, Western blot (Tested During Development)
1.0 mg/ml
V S059
Aqueous buffered solution containing ≤0.09% sodium azide.

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.

Recommended Assay Procedures

Functional Studies: Anti-LECAM-1 monoclonal antibodies have been shown to inhibit neutrophil localization at sites of acute inflammation. Anti-LECAM-1 monoclonal antibody (clone SK11) also induces suppressor function in CD4+ T lymphocytes but does not induce lymphocyte proliferation.

Immunoblotting: Using anti-LECAM-1 monoclonal antibody (clone SK11) in Western blot analysis of extracts from Leu- 8-transfected COS cells and the human T-cell leukemia line (Jurkat) revealed at least seven species of similar molecular weight and at least two closely spaced bands of 76 kD, the largest molecular weight form seen in COS cells.

Product Notices

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  3. Please refer to for technical protocols.
559050 Rev. 7
Antibody Details
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The SK11 monoclonal antibody specifically binds to CD62L which is also known as, L-selectin, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1/LECAM-1), Leukocyte adhesion molecule 1 (LAM1/LAM-1), Lymph node homing receptor (LNHR), Leu-8, or TQ1. CD62L is an ~80 kDa type I transmembrane glycoprotein that belongs to the Selectin/LECAM family. CD62L is differentially expressed on T cells, B cells, monocytes, granulocytes, and a subset of NK cells. The CD62L molecule is the human homolog of the mouse lymph node homing receptor, MEL-14. CD62L plays a role in leucocyte binding to inflamed endothelium and extravasation, as well as mediating lymphocyte homing into peripheral lymphoid tissues through high endothelial postcapillary venules. Soluble CD62L can result from the proteolytic cleavage of cell surface CD62L during cellular activation or inflammation.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

559050 Rev. 7
Format Details
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Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
559050 Rev.7
Citations & References
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Development References (11)

  1. Bevilacqua M, Butcher E, Furie B, et al. Selectins: a family of adhesion receptors. Cell. 1991; 67(2):233. (Biology). View Reference
  2. Camerini D, James SP, Stamenkovic I, Seed B. Leu-8/TQ1 is the human equivalent of the Mel-14 lymph node homing receptor. Nature. 1989; 342(6245):78-82. (Biology). View Reference
  3. Hallmann R, Jutila MA, Smith CW, Anderson DC, Kishimoto TK, Butcher EC. The peripheral lymph node homing receptor, LECAM-1, is involved in CD18-independent adhesion of human neutrophils to endothelium. Biochem Biophys Res Commun. 1991; 174(1):236-243. (Biology). View Reference
  4. Jutila MA, Rott L, Berg EL, Butcher EC. Function and regulation of the neutrophil MEL-14 antigen in vivo: comparison with LFA-1 and MAC-1. J Immunol. 1989; 143(10):3318-3324. (Biology). View Reference
  5. Kanof ME, Strober W, James SP. Induction of CD4 suppressor T cells with anti-Leu-8 antibody. J Immunol. 1987; 139(1):49-54. (Biology). View Reference
  6. Lawrence MB, Springer TA. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell. 1991; 65(5):859-873. (Biology). View Reference
  7. Lewinsohn DM, Bargatze RF, Butcher EC. Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes. J Immunol. 1987; 138(12):4313-4321. (Biology). View Reference
  8. Michie SA, Garcia CF, Strickler JG, Dailey MO, Rouse RV, Warnke RA. Expression of the Leu-8 antigen by B-cell lymphomas. Am J Clin Pathol. 1987; 88(4):486-490. (Biology). View Reference
  9. Picker LJ, Warnock RA, Burns AR, Doerschuk CM, Berg EL, Butcher EC. The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell. 1991; 66(5):921-933. (Biology). View Reference
  10. Polley MJ, Phillips ML, Wayner E, et al. CD62 and endothelial cell-leukocyte adhesion molecule 1 (ELAM-1) recognize the same carbohydrate ligand, sialyl-Lewis x. Proc Natl Acad Sci U S A. 1991; 88(14):6224-6228. (Biology). View Reference
  11. Smith CW, Kishimoto TK, Abbassi O, et al. Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro. J Clin Invest. 1991; 87(2):609-618. (Biology). View Reference
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559050 Rev. 7

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.