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Preparation And Storage
Recommended Assay Procedures
BD® CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and CompBead to ensure that BD® CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
The NK92.39.rMAb monoclonal antibody specifically recognizes human CD96 which is also known as TACTILE (T cell activation increased late expression). CD96 is a type I membrane glycoprotein comprised of three N-terminal Ig-like extracellular domains followed by one mucin-like domain, a transmembrane region, and a cytoplasmic tail with one basic/proline rich motif, a single ITIM motif, and an YXXM motif which is also present in ICOS and CD28. CD96 is expressed at low levels on resting natural killer (NK) cells and T cells and at high levels on activated NK and T cells. CD96 is also expressed at low levels on some B cells and on some T-cell leukemia and acute myeloid leukemia cells. CD96 plays a role in the adhesive interactions of activated NK and T cells with target cells during immune responses. CD96 binds to the poliovirus receptor (CD155) that is highly expressed on some tumor cells. CD155-mediated ligation of CD96 can activate NK cell-mediated cytotoxicity.
Development References (3)
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El-Sherbiny YM, Meade JL, Holmes TD, et al. The requirement for DNAM-1, NKG2D, and NKp46 in the natural killer cell-mediated killing of myeloma cells.. Cancer Res. 2007; 67(18):8444-9. (Clone-specific: Flow cytometry). View Reference
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Fuchs A, Cella M, Giurisato E, Shaw AS, Colonna M. Cutting edge: CD96 (tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155).. J Immunol. 2004; 172(7):3994-8. (Immunogen: Blocking, Flow cytometry). View Reference
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Toutirais O, Cabillic F, Le Friec G, et al. DNAX accessory molecule-1 (CD226) promotes human hepatocellular carcinoma cell lysis by Vgamma9Vdelta2 T cells.. Eur J Immunol. 2009; 39(5):1361-8. (Clone-specific: Flow cytometry, Functional assay). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.