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      Purified Rat Anti-Pig γδ T Lymphocytes
      Purified Rat Anti-Pig γδ T Lymphocytes
      Two-color analysis of pig peripheral blood γδ T lymphocytes. Pig whole blood was stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no.553927, left panel) or purified mAb MAC320 (center and right anels), followed by biotinylated mouse anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553894, all panels) then Streptavidin-PE (Cat. no.554061, all panels). In the right panel, anti-pig γδ TCR mAb PGBL22A (VMRD, Pullman, WA) was added in a fourth staining step, followed by FITC-conjugated rat anti-mouse IgG1 mAb A85-1. Erythrocytes were lysed (PharM Lyse™, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815), and lymphocytes were gated according to their ligh-scatter profile. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA). The right panel demonstrates that MAC320+ cells express the gd TCR and that there are MAC320+ and MAC320- subpopulations of γδ TCR-bearing T lymphocytes in the blood.
      Purified Rat Anti-Pig γδ T Lymphocytes
      Two-color analysis of pig peripheral blood γδ T lymphocytes. Pig whole blood was stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. no.553927, left panel) or purified mAb MAC320 (center and right anels), followed by biotinylated mouse anti-rat IgG2a mAb RG7/1.30 (Cat. no. 553894, all panels) then Streptavidin-PE (Cat. no.554061, all panels). In the right panel, anti-pig γδ TCR mAb PGBL22A (VMRD, Pullman, WA) was added in a fourth staining step, followed by FITC-conjugated rat anti-mouse IgG1 mAb A85-1. Erythrocytes were lysed (PharM Lyse™, Cat. no. 555899), non-viable leukocytes were excluded by staining with 7-AAD (Via-Probe™, Cat. no. 555816/555815), and lymphocytes were gated according to their ligh-scatter profile. Flow cytometry was performed on a FACSCalibur™ (BD Biosciences, San Jose, CA). The right panel demonstrates that MAC320+ cells express the gd TCR and that there are MAC320+ and MAC320- subpopulations of γδ TCR-bearing T lymphocytes in the blood.
      Product Details
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      BD Pharmingen™
      Pig (QC Testing)
      Rat PVG, also known as HO, Black Hooded IgG2a, κ
      Null T cells purified from the blood of young pigs
      Flow cytometry (Routinely Tested), Immunohistochemistry-frozen, Immunoprecipitation (Reported), Cytotoxicity (Not Recommended)
      0.5 mg/ml
      AB_394240
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
      4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      551543 Rev. 1
      Antibody Details
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      MAC320

      The MAC320 antibody monoclonal antibody specifically binds to the 270- and 280-kDa isoforms of the SWC6 heterodimer expressed on Null T cells. These cells form a unique population of CD2-, surface Ig-, and thymus-dependent nylon wool-nonadherent peripheral blood lymphocytes found at high frequencies in young pigs. Null T cells are also CD4- and CD8-, and the majority of them express γδ T-cell receptors (TCR). In addition to peripheral blood, MAC320+ leukocytes (CD2- γδ T cells) are found in the thymus, especially in the medulla and in the marginal zone and red pulp of the spleen. They are rare in normal lymph nodes, tonsils, Peyer's patches, bone marrow, and skin. An additional population of MAC320- CD2+ γδ T cells is found in the blood and spleen. The MAC320 antibody does not react with pig erythrocytes, monocytes, or granulocytes, nor with cow, sheep, or human lymphocytes.

      551543 Rev. 1
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      551543 Rev.1
      Citations & References
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      View product citations for antibody "551543" on CiteAb

      Development References (5)

      1. Binns RM, Duncan IA, Powis SJ, Hutchings A, Butcher GW. Subsets of null and gamma delta T-cell receptor+ T lymphocytes in the blood of young pigs identified by specific monoclonal antibodies. Immunology. 1992 October; 77(2):219-227. (Immunogen: Cytotoxicity, Immunoprecipitation). View Reference
      2. Binns RM, Whyte A, Licence ST. The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs. J Immunol. 1996; 157(9):4094-4099. (Clone-specific: Immunohistochemistry). View Reference
      3. Binns RM. The Null/gamma delta TCR+ T cell family in the pig. Vet Immunol Immunopathol. 1994 October; 43(1-3):69-77. (Biology). View Reference
      4. Davis WC, Zuckermann FA, Hamilton MJ, et al. Analysis of monoclonal antibodies that recognize gamma delta T/null cells. Vet Immunol Immunopathol. 1998 January; 60(3-4):305-316. (Biology). View Reference
      5. Yang H, Parkhouse RM. Phenotypic classification of porcine lymphocyte subpopulations in blood and lymphoid tissues. Immunology. 1996; 89(1):76-83. (Biology). View Reference
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      551543 Rev. 1

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.