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      Purified Rat Anti-Mouse PIR-A/B
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      This SKU will be discontinuing Apr 2024. For additional support, contact your local applications specialist. Contact Us #
      Purified Rat Anti-Mouse PIR-A/B
      The expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and stained with purified 6C1 monoclonal antibody (shaded histogram) followed by biotin-conjugated RG11/39.4 mAb (anti-rat IgG1, Cat. no. 553890, both histograms), then Streptavidin-PE (Cat. no. 554061, both histograms). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
      Purified Rat Anti-Mouse PIR-A/B
      The expression of PIR-A/B on mouse spleen cells. BALB/c splenocytes were preincubated with Mouse BD Fc Block™ (Cat. no. 553141/553142) and stained with purified 6C1 monoclonal antibody (shaded histogram) followed by biotin-conjugated RG11/39.4 mAb (anti-rat IgG1, Cat. no. 553890, both histograms), then Streptavidin-PE (Cat. no. 554061, both histograms). Flow cytometry was performed on a BD FACScan™ Flow Cytometry System.
      Product Details
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      BD Pharmingen™
      Mouse (QC Testing)
      Rat LEW, also known as Lewis IgG1, κ
      Recombinant mouse PIR-A1 extracellular domain peptide and the mouse myeloid cell line, WEH13
      Flow cytometry (Routinely Tested), Immunoprecipitation (Reported)
      0.5 mg/ml
      AB_393627
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
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      Recommended Assay Procedures

      Mouse BD Fc Block™ (Cat. no. 553141/553142) should be used when staining with the 6C1 antibody, and a second-step antibody which does not cross-react with Mouse BD Fc Block™ (mAb 2.4G2, rat IgG2b, κ) must be used. We recommend biotinylated anti-rat IgG1 mAb RG11/39.4 (Cat. no. 553890) followed by a "bright" third-step reagent, such as Streptavidin-PE (Cat. no. 554061) for optimal staining. Other reported applications include immunoprecipitation.  

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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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      550348 Rev. 5
      Antibody Details
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      6C1

      The 6C1 monoclonal antibody specifically recognizes the common epitopes of PIR-A and PIR-B (Paired Immunoglobulin-liked Receptors) in all mouse strains tested (A/J, BALB/cJ, C3H, C57BL/6, DBA/1, DBA/2, NZB, and SJL).  PIR-A and PIR-B are type-I transmembrane glycoproteins containing six Ig-like domains.  There are multiple PIR-A proteins which are activating receptors by virtue of their intracellular association with the ITAM (Immunoreceptor Tyrosine-based Activation Motif)- containing Fc receptor γ chain (FcRγ) in mast cells  and macrophages.  FcRγ expression is required for PIR-A cell-surface expression on dendritic cells, B cells, and myeloid lineages.  In contrast, PIR-B is an inhibitory receptor which contains various ITIMs (Immunoreceptor Tyrosine-based Inhibitory Motifs) in its cytoplasmic domain.  These receptors are expressed on B cells, granulocytes, mast cells, dendritic cells, and monocytes/macrophages, but not on thymocytes, T lymphocytes, erythroid lineage cells, or NK cells.  The level of cell-surface expression of these receptors increases as a function of B-cell activation and myeloid- and B-lineage differentiation.  The 6C1 antibody immunoprecipitates molecules of 85- and 125-kDa, which correspond to PIRA and PIR-B, respectively.  

      550348 Rev. 5
      Format Details
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      Purified
      Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
      Purified
      550348 Rev.5
      Citations & References
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      Development References (6)

      1. Bléry M, Kubagawa H, Chen CC, Vély F, Cooper MD, Vivier E. The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1. Proc Natl Acad Sci U S A. 1998; 95(5):2446-2451. (Biology). View Reference
      2. Chen CC, Hurez V, Brockenbrough JS, Kubagawa H, Cooper MD. Paternal monoallelic expression of the paired immunoglobulin-like receptors PIR-A and PIR-B. Proc Natl Acad Sci U S A. 1999; 96(12):6868-6872. (Clone-specific). View Reference
      3. Kubagawa H, Burrows PD, Cooper MD. A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells. Proc Natl Acad Sci U S A. 1997; 94(10):5261-5266. (Biology). View Reference
      4. Kubagawa H, Chen CC, Ho LH. Biochemical nature and cellular distribution of the paired immunoglobulin-like receptors, PIR-A and PIR-B. J Exp Med. 1999; 189(2):309-318. (Immunogen: Immunoprecipitation). View Reference
      5. Maeda A, Kurosaki M, Kurosaki T. Paired immunoglobulin-like receptor (PIR)-A is involved in activating mast cells through its association with Fc receptor gamma chain. J Exp Med. 1998; 188(5):991-995. (Biology). View Reference
      6. Taylor LS, McVicar DW. Functional association of FcepsilonRIgamma with arginine(632) of paired immunoglobulin-like receptor (PIR)-A3 in murine macrophages. Blood. 1999; 94(5):1790-1796. (Biology). View Reference
      View All (6) View Less
      550348 Rev. 5

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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