Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Portugal (English)
Profile Icon Profile Icon
Sign-in/Register UserName
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
      Alert Icon
      PE Rat Anti-Mouse IL-13
      PE Rat Anti-Mouse IL-13
      Multiparameter flow cytometric analysis of IL-13 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e (Cat. No. 553057/567114/567115; 10 μg/ml for coating) and soluble Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294/567110) antibodies with Recombinant Mouse IL-2 (Cat. No. 550069; 10 ng/ml) and Recombinant Mouse IL-4 (Cat. No. 550067; 25 ng/ml) proteins. The cells were subsequently cultured (3 days) with Recombinant IL-2 and IL-4 and then stimulated with PMA (Sigma Cat. No. P-8139) and Ionomycin (Sigma Cat. No. I-0634) in the presence of BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) [Cat. No. 554724] for 5.5 hours. The cells were washed and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The fixed cells were washed and then permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with APC Rat Anti-Mouse CD4 antibody (Cat. No. 561091/553051) and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse IL-13 antibody (Cat. No. 568551; Right Plot) at 0.06 µg/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 (or Ig Isotype control staining) versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      PE Rat Anti-Mouse IL-13
      Multiparameter flow cytometric analysis of IL-13 expression in stimulated mouse T cells. Mouse splenic CD4+ T cells were cultured (2 days) with plate-bound Purified NA/LE Hamster Anti-Mouse CD3e (Cat. No. 553057/567114/567115; 10 μg/ml for coating) and soluble Purified NA/LE Hamster Anti-Mouse CD28 (Cat. No. 553294/567110) antibodies with Recombinant Mouse IL-2 (Cat. No. 550069; 10 ng/ml) and Recombinant Mouse IL-4 (Cat. No. 550067; 25 ng/ml) proteins. The cells were subsequently cultured (3 days) with Recombinant IL-2 and IL-4 and then stimulated with PMA (Sigma Cat. No. P-8139) and Ionomycin (Sigma Cat. No. I-0634) in the presence of BD GolgiStop™ Protein Transport Inhibitor (containing Monensin) [Cat. No. 554724] for 5.5 hours. The cells were washed and fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The fixed cells were washed and then permeabilized and stained in BD Perm/Wash™ Buffer (Cat. No. 554723) with APC Rat Anti-Mouse CD4 antibody (Cat. No. 561091/553051) and with either PE Rat IgG1, κ Isotype Control (Cat. No. 553925; Left Plot) or PE Rat Anti-Mouse IL-13 antibody (Cat. No. 568551; Right Plot) at 0.06 µg/test using the BD Biosciences Intracellular Cytokine Staining protocol. The bivariate pseudocolor density plot showing the correlated expression of IL-13 (or Ig Isotype control staining) versus CD4 was derived from gated events with the forward and side light-scatter characteristics of intact stimulated lymphocytes. Flow cytometry and data analysis were performed using a BD LSRFortessa™ Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
      Product Details
      Down Arrow Up Arrow


      BD Pharmingen™
      Il-13; Il13; interleukin-13
      Mouse (QC Testing)
      Rat WI, also known as Wistar (outbred) IgG1, κ
      Mouse IL-13 Recombinant Protein
      Intracellular staining (flow cytometry) (Routinely Tested)
      0.2 mg/ml
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions and unconjugated antibody and free dye were removed.
      Show More blue down arrow Show Less blue up arrow

      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation).  When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells.   However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls.  It is strongly recommended that when using a reagent for the first time, users compare the spillover on cell and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

      Show More blue down arrow Show Less blue up arrow

      Product Notices

      1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      6. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      Show More blue down arrow Show Less blue up arrow
      568551 Rev. 1
      Antibody Details
      Down Arrow Up Arrow
      W19-895

      The W19-895 monoclonal antibody specifically recognizes mouse Interleukin-13 (IL-13), which is encoded by Il13 (interleukin 13). IL-13 is a multifunctional immunoregulatory cytokine that shares many functional properties with IL-4. It functions as a key regulator of immune responses against parasitic infections and as a mediator of inflammation involved in asthma and allergies. IL-13 is secreted by T helper 2 (Th2)-like cells, NK cells, Th1-like cells, CD8+ T cells, Innate lymphoid type 2 cells (ILC2), mast cells, macrophages, monocytes, basophils, and eosinophils. IL-13 promotes B-cell proliferation and induces immunoglobulin class switching to IgE and expression of CD23 (FcϵRII). It also upregulates expression of β2 integrin family members CD11b, CD11c, CD18, and induces MHC class II and CD23 expression on monocytes and macrophages. IL-13 directs the functions of these cells as well as endothelial cells, smooth muscle cells, fibroblasts, and epithelial cells by regulating their production of pro-inflammatory mediators including IL-1, IL-6, IL-12, TNF, and prostaglandins and reactive oxygen and nitrogen intermediates. IL-13 is the central mediator of allergic asthma in lungs where it regulates eosinophilic inflammation, mucus secretion, and airway hyperresponsiveness. IL-13 and IL-4 can bind with high affinity and signal intracellularly through the cell surface Type 2 IL-4 Receptor Complex comprised of IL-13 R alpha 1 (CD213a) and IL-4R alpha (CD124) chains. IL-13 also binds with high affinity to cell surface or soluble forms of the IL-13 R alpha 2 (CD213a2) which prevents IL-13 from signaling through the Type 2 IL-4 Receptor Complex and thus controls the bioavailability of IL-13.

      568551 Rev. 1
      Format Details
      Down Arrow Up Arrow
      PE
      R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      PE
      Yellow-Green 488 nm, 532 nm, 561 nm
      496 nm, 566 nm
      576 nm
      568551 Rev.1
      Citations & References
      Down Arrow Up Arrow
      View product citations for antibody "568551" on CiteAb

      Development References (11)

      1. Brown KD, Zurawski SM, Mosmann TR, Zurawski G. A family of small inducible proteins secreted by leukocytes are members of a new superfamily that includes leukocyte and fibroblast-derived inflammatory agents, growth factors, and indicators of various activation processes.. J Immunol. 1989; 142(2):679-87. (Biology). View Reference
      2. Cherwinski HM, Schumacher JH, Brown KD, Mosmann TR. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987; 166(5):1229-1244. (Biology). View Reference
      3. Daines MO, Hershey GK. A novel mechanism by which interferon-gamma can regulate interleukin (IL)-13 responses. Evidence for intracellular stores of IL-13 receptor alpha -2 and their rapid mobilization by interferon-gamma.. J Biol Chem. 2002; 277(12):10387-93. (Biology). View Reference
      4. Doherty TM, Kastelein R, Menon S, Andrade S, Coffman RL.. Modulation of murine macrophage function by IL-13. J Immunol. 1993; 151(12):7151-7160. (Biology). View Reference
      5. Hershey GK. IL-13 receptors and signaling pathways: an evolving web.. J Allergy Clin Immunol. 2003; 111(4):677-90; quiz 691. (Biology). View Reference
      6. Iwaszko M, Biały S, Bogunia-Kubik K. Significance of Interleukin (IL)-4 and IL-13 in Inflammatory Arthritis.. Cells. 2021; 10(11):3000. (Biology). View Reference
      7. McKenzie A, Zurawski G. Measurement of IL-13. In: Coligan, Kruisbeek, Shevak, Strober, ed. Current Protocols in Immunology. New York: John Wiley & Sons; 1994:18-19.
      8. McKenzie AN, Culpepper JA, de Waal Malefyt R, et al. Interleukin 13, a T-cell-derived cytokine that regulates human monocyte and B-cell function.. Proc Natl Acad Sci U S A. 1993; 90(8):3735-9. (Biology). View Reference
      9. McKenzie GJ, Fallon PG, Emson CL, Grencis RK, McKenzie AN. Simultaneous disruption of interleukin (IL)-4 and IL-13 defines individual roles in T helper cell type 2-mediated responses.. J Exp Med. 1999; 189(10):1565-72. (Biology). View Reference
      10. Wynn TA. IL-13 effector functions.. Annu Rev Immunol. 2003; 21:425-56. (Biology). View Reference
      11. Zurawski G, de Vries JE.. Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells. Immunol Today. 1994; 15(1):19-26. (Biology). View Reference
      View All (11) View Less
      568551 Rev. 1

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.