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FITC Rat Anti-Mouse CD71
FITC Rat Anti-Mouse CD71
Two-color analysis of the expression of CD71 on developing erythroid cells. C57BL/6 bone-marrow leukocytes were simultaneously stained with PE-conjugated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553673) and FITC- conjugated mAb C2 (Right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Two-color analysis of the expression of CD71 on developing erythroid cells. C57BL/6 bone-marrow leukocytes were simultaneously stained with PE-conjugated anti-mouse Erythroid Cells mAb TER-119 (Cat. no. 553673) and FITC- conjugated mAb C2 (Right panel). Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
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BD Pharmingen™
Transferrin Receptor; TR; TfR; TfR1; Tfrc; Trfr; Mtvr-1
Mouse (QC Testing)
Rat WF, also known as Wistar Furth IgG1, κ
Mouse cell line
Flow cytometry (Routinely Tested)
0.5 mg/ml
22042
AB_394743
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
553266 Rev. 8
Antibody Details
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C2

The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor. CD71 is a disulfide-linked homodimer of 95-kDa subunits. CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements. It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes. The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin.

553266 Rev. 8
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
553266 Rev.8
Citations & References
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View product citations for antibody "553266" on CiteAb

Development References (4)

  1. Kemp JD, Thorson JA, Gomez F, Smith KM, Cowdery JS, Ballas ZK. Inhibition of lymphocyte activation with anti-transferrin receptor Mabs: a comparison of three reagents and further studies of their range of effects and mechanism of action. Cell Immunol. 1989; 122(1):218-230. (Clone-specific: Activation). View Reference
  2. Kemp JD, Thorson JA, McAlmont TH, Horowitz M, Cowdery JS, Ballas ZK. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol. 1987; 138(8):2422-2426. (Immunogen: Activation). View Reference
  3. Lok CN, Loh TT. Regulation of transferrin function and expression: review and update. Biol Signals Recept. 1998; 7(3):157-178. (Biology). View Reference
  4. Thorson JA, Smith KM, Gomez F, Naumann PW, Kemp JD. Role of iron in T cell activation: TH1 clones differ from TH2 clones in their sensitivity to inhibition of DNA synthesis caused by IgG Mabs against the transferrin receptor and the iron chelator deferoxamine. Cell Immunol. 1991; 134(1):126-137. (Clone-specific: Activation). View Reference
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553266 Rev. 8

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.