BV510 Mouse Anti-Human CD93

BD OptiBuild™ BV510 Mouse Anti-Human CD93

Name: BV510 Mouse Anti-Human CD93
Brand: BV510 Mouse Anti-Human CD93
SKU: 743196

Clone R139 (RUO)

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Product Details

Brand: BD OptiBuild™

Alternative Name: C1QR1; C1qRP; C1qR(P); C1q/MBL/SPA Receptor; MXRA4; GR11; Dj737e23.1

Reactivity: Human (Tested in Development)

Isotype: Mouse BALB/c IgG2b, κ

Immunogen: Clq-CLF-binding proteins

Application: Flow cytometry (Qualified)

Concentration: 0.2 mg/ml

RRID: AB_2741335

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes (including BD OptiBuild Brilliant reagents) are used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD Horizon Brilliant Stain Buffer (Cat. No. 563794).

Product Notices

This antibody was developed for use in flow cytometry.
The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
Researchers should determine the optimal concentration of this reagent for their individual applications.
An isotype control should be used at the same concentration as the antibody of interest.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
BD Horizon Brilliant Stain Buffer is covered by one or more of the following US patents: 8,110,673; 8,158,444; 8,575,303; 8,354,239.
BD Horizon Brilliant Violet 510 is covered by one or more of the following US patents: 8,575,303; 8,354,239.

Companion Products

Human BD Fc Block™ RUO

Size 50 µg Cat No. 564219

Stain Buffer (FBS) RUO

Size 500 ML Cat No. 554656

Stain Buffer (BSA) RUO

Size 500 ML Cat No. 554657

Brilliant Stain Buffer RUO

Size 100 Tests Cat No. 563794

Lysing Buffer RUO

Size 100 ML Cat No. 555899

Lysing Solution 10X Concentrate IVD

Size 100 ML Cat No. 349202

View Details

743196 Rev. 2

Antibody Details

R139

The R139 monoclonal antibody specifically binds to CD93 which is also known as Complement component C1q receptor (C1qR), C1q receptor 1 (C1qR1), or Matrix-remodeling-associated protein 4 (MXRA4). The immunogen used to generate the R139 hybridoma was a preparation of CD93 protein. Human CD93 is a transmembrane glycoprotein that is highly expressed on monocytes, macrophages, granulocytes, and endothelial cells but not on T and B lymphocytes. CD93 is also known as the C1q/MBL/SPA Receptor as it binds C1q, the recognition subunit of the first component (C1) of the complement pathway, as well as MBL (Mannose-binding-lectin) and SPA (Pulmonary Surfactant Protein A). Human C1qRp is involved in the C1q-mediated enhancement of phagocytosis. R139 is suitable to detect CD93 expression on cells of myeloid lineage by flow cytometry, and CD93 in cellular lysates by Western blotting or immunoprecipitation. In addition, R139 reportedly neutralizes C1q-mediated enhancement of phagocytosis. CD93 has also been reported to define a human stem cell population with hematopoietic and hepatic potential.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

743196 Rev. 2

Format Details

BV510

The BD Horizon Brilliant Violet™ 510 (BV510) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye with an excitation maximum (Ex Max) at 327-nm / 405-nm and an emission maximum (Em Max) at 512-nm. BV510, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 510-nm (e.g., a 525/50 bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: BV510

Excitation Max: 327 nm, 405 nm

Emission Max: 512 nm

743196 Rev.2

Citations & References

View product citations for antibody "743196" on CiteAb

Development References (7)

Danet GH, Luongo JL, Butler G, et al. C1qRp defines a new human stem cell population with hematopoietic and hepatic potential.. Proc Natl Acad Sci USA. 2002; 99(16):10441-5. (Biology). View Reference
Guan E, Robinson SL, Goodman EB, Tenner AJ. Cell-surface protein identified on phagocytic cells modulates the C1q-mediated enhancement of phagocytosis. J Immunol. 1994; 152(8):4005-4016. (Immunogen: Bioassay, ELISA, Flow cytometry, Immunoprecipitation, Inhibition, Neutralization, Western blot). View Reference
Guan EN, Burgess WH, Robinson SL, Goodman EB, McTigue KJ, Tenner AJ. Phagocytic cell molecules that bind the collagen-like region of C1q. Involvement in the C1q-mediated enhancement of phagocytosis. J Biol Chem. 1991; 266(30):20345-20355. (Immunogen: Functional assay, Immunoprecipitation, Inhibition, Western blot). View Reference
Nepomuceno RR, Henschen-Edman AH, Burgess WH, Tenner AJ. cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro. Immunity. 1997; 6(2):119-129. (Clone-specific: Western blot). View Reference
Nepomuceno RR, Ruiz S, Park M, Tenner AJ. C1qRP is a heavily O-glycosylated cell surface protein involved in the regulation of phagocytic activity. J Immunol. 1999; 162(6):3583-3589. (Clone-specific: Flow cytometry, Functional assay, Immunoprecipitation, Inhibition). View Reference
Nepomuceno RR, Tenner AJ. C1qRP, the C1q receptor that enhances phagocytosis, is detected specifically in human cells of myeloid lineage, endothelial cells, and platelets. J Immunol. 1998; 160(4):1929-1935. (Clone-specific: Flow cytometry, Immunoprecipitation). View Reference
Tenner AJ. C1q receptors: regulating specific functions of phagocytic cells. Immunobiology. 1998; 199(2):250-264. (Biology). View Reference

View All (7)

743196 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.