BD Pharmingen™ Biotin Mouse Anti-Human CD24
Clone ML5 (RUO)
Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415; Top Plots) and with either Biotin Mouse IgG2a, κ Isotype Control (Cat. No. 553455; Left Plots) or Biotin Mouse Anti-Human CD24 antibody (Cat. No. 567713; Right Plots) at 1.0 µg/test. The cells were then counterstained with BD Horizon™ BV421 Streptavidin (Cat. No. 563259). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plots showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 (Top Plots) or side-light scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Multiparameter flow cytometric analysis of CD24 expression on Human peripheral blood leucocyte populations. Human whole blood was stained with APC Mouse Anti-Human CD19 antibody (Cat. No. 555415; Top Plots) and with either Biotin Mouse IgG2a, κ Isotype Control (Cat. No. 553455; Left Plots) or Biotin Mouse Anti-Human CD24 antibody (Cat. No. 567713; Right Plots) at 1.0 µg/test. The cells were then counterstained with BD Horizon™ BV421 Streptavidin (Cat. No. 563259). Erythrocytes were lysed with BD FACS Lysing Solution (Cat. No. 349202). The bivariate pseudocolor density plots showing the correlated expression of CD24 (or Ig Isotype control staining) versus CD19 (Top Plots) or side-light scatter (SSC-A) signals (Bottom Plots) were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ X-20 Cell Analyzer System. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Companion Products
The ML5 monoclonal antibody specifically binds to CD24 which is also known as CD24A, signal transducer CD24, small cell lung carcinoma cluster 4 antigen, or BA-1. CD24 is a 35-70 kDa glycophosphatidylinositol (GPI)-linked glycoprotein whose glycosylation pattern is highly variable and cell-type dependent. CD24 is expressed on neutrophils, eosinophils, dendritic cells, neural cells, epithelial cells, muscle cells, and many types of cancer cells. CD24 functions as an adhesion receptor. Several different ligands have been identified for CD24 including CD62P (P-selectin) which is expressed on activated platelets and activated endothelium. CD24 is variably expressed on all B lineage cells, except plasma cells, and can play a role in regulating the activation, proliferation or differentiation of these cells.
Development References (6)
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Chtanova T, Tangye SG, Newton R, et al. T follicular helper cells express a distinctive transcriptional profile, reflecting their role as non-Th1/Th2 effector cells that provide help for B cells. J Immunol. 2004; 173(1):68-78. (Clone-specific: Flow cytometry). View Reference
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Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD24. In: Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:82-83.
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McMichael AJ. A.J. McMichael .. et al., ed. Leucocyte typing III : white cell differentiation antigens. Oxford New York: Oxford University Press; 1987:1-1050.
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Salamone MC, Rosselot C, Salamone GV, Barboza M, Kado M, Fainboim L. Antibodies recognizing CD24 LAP epitope on human T cells enhance CD28 and IL-2 T cell proliferation . J Leukoc Biol. 2001; 69(2):215-223. (Clone-specific: Flow cytometry). View Reference
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Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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Zola H. Leukocyte and stromal cell molecules : the CD markers. Hoboken, N.J.: Wiley-Liss; 2007.
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.