Preparation And Storage
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Companion Products
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Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docked state and/or membrane fusion. VTI1a and VTI1b are putative mammalian SNARE proteins identified by sequence comparison with yeast SNAREs. In line with their involvement in vesicle transport, these molecules are expressed in a wide range of mammalian tissues. Vti1a, a possible t-SNARE, contains a C-terminal hydrophobic domain and several regions that may form coiled-coil structures. It exists in distinct syntaxin 5- and syntaxin 6-containing SNARE complexes within the Golgi apparatus. Inhibition of Vti1a blocks transport of G proteins to the cell surface and results in their accumulation within the Golgi. Thus, Vti1a functions in protein transport within the secretory pathway.
Development References (5)
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Advani RJ, Bae HR, Bock JB, et al. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J Biol Chem. 1998; 273(17):10317-10324. (Biology). View Reference
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Chiu R, Novikov L, Mukherjee S, Shields D. A caspase cleavage fragment of p115 induces fragmentation of the Golgi apparatus and apoptosis. J Biol Chem. 2002; 159(4):637-648. (Clone-specific: Western blot). View Reference
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Mallard F, Tang BL, Galli T. Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform. J Cell Biol. 2002; 156(4):653-664. (Clone-specific: Western blot). View Reference
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Shorter J, Beard MB, Seemann J, Dirac-Svejstrup AB, Warren G. Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115. J Cell Biol. 2002; 157(1):45-62. (Clone-specific: Western blot). View Reference
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Xu Y, Wong SH, Tang BL, Subramaniam VN, Zhang T, Hong W. A 29-kilodalton Golgi soluble N-ethylmaleimide-sensitive factor attachment protein receptor (Vti1-rp2) implicated in protein trafficking in the secretory pathway. J Biol Chem. 1998; 273(34):21783-21789. (Biology). View Reference
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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.