Preparation And Storage
Recommended Assay Procedures
ELISA Detection: The biotinylated XMG1.2 clone is useful as a detection antibody in a sandwich ELISA for measuring mouse IFN-γ protein levels. Biotinylated XMG1.2 antibody can be paired with the purified R4-6A2 antibody (Cat. No. 551216) or purified AN-18 (Cat. No. 551309) as the capture antibody, with recombinant mouse IFN-γ protein (Cat. No. 554587) as the standard. The biotinylated XMG1.2 antibody preparation should be titrated from 0.5-2.0 µg/ml to determine its optimal concentration for ELISA detection. To obtain linear standard curves, doubling dilutions of IFN-γ protein ranging from ~4000 to 30 pg/ml are recommended for inclusion in each ELISA plate. Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. For testing IFN-γ in serum or plasma, our BD OptEIA™ Set (Cat. No. 551866) or BD OptEIA™ Kit (Cat. No. 558258) are recommended.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
The XMG1.2 monoclonal antibody specifically binds to mouse interferon-γ (IFN-γ) protein. IFN-γ is a pleiotropic cytokine, of approximately 15-17 kDa, involved in the regulation of inflammatory and immune responses. It plays an important role in activation, growth, and differentiation of T and B lymphocytes, macrophages, NK cells and other non-hematopoietic cell types. IFN-γ production is associated with the Th1 cell differentiation. The purified form of this antibody has been reported to be a neutralizing antibody.
Development References (3)
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Abrams JS, Roncarolo MG, Yssel H, Andersson U, Gleich GJ, Silver JE. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 1992; 127:5-24. (Clone-specific). View Reference
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Cherwinski HM, Schumacher JH, Brown KD, Mosmann TR. Two types of mouse helper T cell clone. III. Further differences in lymphokine synthesis between Th1 and Th2 clones revealed by RNA hybridization, functionally monospecific bioassays, and monoclonal antibodies. J Exp Med. 1987; 166(5):1229-1244. (Clone-specific). View Reference
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Sander B, Hoiden I, Andersson U, Moller E, Abrams JS. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Methods. 1993; 166(2):201-214. (Clone-specific). View Reference
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For Research Use Only. Not for use in diagnostic or therapeutic procedures.