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RB744 Rat Anti-Mouse CD88 (C5a Receptor)
Product Details
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BD OptiBuild™
Cd88; C5ar1; C5a-R; C5aR; C5r1; C5a Ligand; C5a anaphylatoxin receptor
Mouse (Tested in Development)
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG2b, κ
Mouse C5aR Transfected Cell Line
Flow cytometry (Qualified)
0.2 mg/ml
12273
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

  1. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  2. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
  3. For U.S. patents that may apply, see bd.com/patents.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  6. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
  7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
  8. When using high concentrations of antibody, background binding of this dye to erythroid fragments produced by ammonium chloride-based lysis, such as with BD Pharm Lyse™ Lysing Buffer (Cat. No. 555899), has been observed when the antibody conjugate was present during the lysis procedure. This may cause nonspecific staining of target cells, such as leukocytes, which have bound the resulting erythroid fragments. This background can be mitigated by any of the following: titrating the antibody conjugate to a lower concentration, fixing samples with formaldehyde, or removing erythrocytes before staining (eg, gradient centrifugation or pre-lysis with wash). This background has not been observed when cells were lysed with BD FACS™ Lysing Solution (Cat. No. 349202) after staining.
  9. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  10. An isotype control should be used at the same concentration as the antibody of interest.
  11. Please observe the following precautions: We recommend that special precautions be taken (such as wrapping vials, tubes, or racks in aluminum foil) to protect exposure of conjugated reagents, including cells stained with those reagents, to any room illumination. Absorption of visible light can significantly affect the emission spectra and quantum yield of tandem fluorochrome conjugates.
757217 Rev. 1
Antibody Details
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20/70

The 20/70 monoclonal antibody specifically binds to CD88, which is also known as, Complement component 5a receptor 1 (C5ar1), C5a anaphylatoxin chemotactic receptor, or C5a receptor (C5aR). CD88 belongs to the rhodopsin family of seven-transmembrane G protein-coupled receptors and mediates its effects by binding to the complement activation product, C5a. CD88 is expressed on leucocytes including, neutrophils, monocytes, macrophages, and eosinophils. CD88 may also be expressed on alveolar epithelial cells, endothelial cells, neural stem cells, oligodendrocytes, and on parenchymal cells of lung, liver, kidney and heart. Binding of the proinflammatory C5a anaphylatoxin to CD88 results in G-protein coupling and intracellular signal transduction that mediates intracellular calcium release, chemotaxis, degranulation, cytokine production, and superoxide anion production. The 20/70 antibody can block binding of C5a to CD88 and thus inhibit its biological functions.

757217 Rev. 1
Format Details
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RB744
The BD Horizon RealBlue™ 744 (RB744) Dye is part of the BD® family of blue dyes. It is a tandem fluorochrome with an excitation maximum (Ex Max) at 498-nm and an emission maximum (Em Max) at 746-nm as measured using an antibody-dye conjugate. Driven by BD® innovation, RB744 can be used on both spectral and conventional cytometers and is designed to be excited by the Blue laser (488-nm) with minimal excitation by the 561-nm Yellow-Green laser. For conventional instruments equipped with a Blue laser (488-nm), we recommend using an optical filter centered near 750-nm (e.g., a 750/60-nm bandpass filter).
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RB744
Blue 488 nm
498 nm
746 nm
757217 Rev.1
Citations & References
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View product citations for antibody "757217" on CiteAb

Development References (6)

  1. Godau J, Heller T, Hawlisch H, et al. C5a initiates the inflammatory cascade in immune complex peritonitis.. J Immunol. 2004; 173(5):3437-45. (Clone-specific: Blocking, Flow cytometry, Inhibition, In vivo exacerbation). View Reference
  2. Laudes IJ, Chu JC, Huber-Lang M, et al. Expression and function of C5a receptor in mouse microvascular endothelial cells. J Immunol. 2002; 169(10):5962-5970. (Biology). View Reference
  3. Lee H, Whitfeld PL, Mackay CR. Receptors for complement C5a. The importance of C5aR and the enigmatic role of C5L2. Immunol Cell Biol. 2008; 86(2):153-160. (Biology). View Reference
  4. Nataf S, Levison SW, Barnum SR. Expression of the anaphylatoxin C5a receptor in the oligodendrocyte lineage. Brain Res. 2001; 894(2):321-326. (Biology). View Reference
  5. Riedemann NC, Guo RF, Neff TA, et al. Increased C5a receptor expression in sepsis. J Clin Invest. 2002; 110(1):101-108. (Biology). View Reference
  6. Soruri A, Kim S, Kiafard Z, Zwirner J. Characterization of C5aR expression on murine myeloid and lymphoid cells by the use of a novel monoclonal antibody. Immunol Lett. 2003; 88(1):47-52. (Immunogen: Blocking, Flow cytometry, Functional assay, Inhibition). View Reference
View All (6) View Less
757217 Rev. 1

 

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For Research Use Only. Not for use in diagnostic or therapeutic procedures.