BD Horizon™ R718 Rat Anti-Mouse Blimp-1
Clone 5E7 (RUO)
Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Left Plot) or BD Horizon™ R718 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567845; Right Plot) at 0.125 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Left Plot) or BD Horizon™ R718 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567845; Right Plot) at 0.125 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Left Plot) or BD Horizon™ R718 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567845; Right Plot) at 0.125 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Multicolor flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes. Mouse splenic leucocytes were stimulated for 3 days with lipopolysaccharide LPS) and stained with BD Horizon™ BV421 Rat Anti-Mouse CD45R/B220 antibody (Cat. No. 562922). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either BD Horizon™ R718 Rat IgG2a, κ Isotype Control (Cat. No. 566941; Left Plot) or BD Horizon™ R718 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 567845; Right Plot) at 0.125 µg/test. The bivariate pseudocolor density plot showing the correlated expression of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 was derived from gated events with the forward and side light-scatter characteristics of intact activated leucocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype Control-stained cells (data not shown). Flow cytometry and data analysis were performed using a BD LSRFortessa™ X-20 Cell Analyzer System and FlowJo™ software. Data shown on this Technical Data Sheet are not lot specific.
Preparation And Storage
Recommended Assay Procedures
BD™ CompBeads can be used as surrogates to assess fluorescence spillover (Compensation). When fluorochrome conjugated antibodies are bound to BD CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD CompBead to ensure that BD CompBeads are appropriate for your specific cellular application.
Product Notices
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
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Companion Products
The 5E7 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1). Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene and functions as a transcriptional repressor. Blimp-1 is essential for regulating the terminal differentiation of activated B cells into plasma cells. In this process, Blimp-1 suppresses genes involved in B cell proliferation, such as Myc and Bcl6. It controls other genes, such as Xbp1, that are required for upregulating the plasma cell's protein synthesis machinery which is essential for antibody production and secretion. Blimp-1 is an important regulator in other cell types as well. It functions in the regulation of T cell activation and homeostasis and NK cell maturation. Blimp-1 is expressed in activated T cells and can function to inhibit IL-2 production while enhancing the differentiation and expression of several molecules produced by effector T cells.
Development References (9)
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Crotty S, Johnston RJ, Schoenberger SP. Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation. Nat Immunol. 2010; 11(2):114-120. (Biology). View Reference
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Huang S. Blimp-1 is the murine homolog of the human transcriptional repressor PRDI-BF1. Cell. 1994; 78(1):9. (Biology). View Reference
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Johnston RJ, Poholek AC, DiToro D, et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.. Science. 2009; 325(5943):1006-10. (Biology). View Reference
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Kallies A, Carotta S, Huntington ND, et al. A role for Blimp1 in the transcriptional network controlling natural killer cell maturation. Blood. 2011; 117(6):1869-1879. (Immunogen: Western blot). View Reference
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Kallies A, Hasbold J, Tarlinton DM, et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med. 2004; 200(8):967-977. (Biology). View Reference
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Kallies A, Hawkins ED, Belz GT, et al. Transcriptional repressor Blimp-1 is essential for T cell homeostasis and self-tolerance. Nat Immunol. 2006; 7(5):466-474. (Biology). View Reference
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Kim SJ, Gregersen PK, Diamond B. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest. 2013; 123(2):823-833. (Biology). View Reference
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Martins GA, Cimmino L, Liao J, Magnusdottir E, Calame K. Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival. J Exp Med. 2008; 205(9):1959-1965. (Biology). View Reference
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Turner CA Jr, Mack DH, Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell. 1994; 77(2):297-306. (Biology). View Reference
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.