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      R718 Rat Anti-Human CD132
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      If you're planning to order more than 10 BD OptiBuild™ Reagents, please contact your local sales representative to place this order. Contact Us #
      R718 Rat Anti-Human CD132
      Flow cytometric analysis using BD OptiBuild™ R718 Rat Anti-Human CD132 antibody (Cat. No. 752291; solid line histogram) on Human peripheral blood lymphocytes, with IgG Isotype Control (Cat. No. 566943; dashed line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
      R718 Rat Anti-Human CD132
      Flow cytometric analysis using BD OptiBuild™ R718 Rat Anti-Human CD132 antibody (Cat. No. 752291; solid line histogram) on Human peripheral blood lymphocytes, with IgG Isotype Control (Cat. No. 566943; dashed line histogram). Flow cytometry was performed using a BD LSRFortessa™ X-20  Flow Cytometer System.
      Product Details
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      BD OptiBuild™
      IL-2RG; IL-2Rγ; γc; Common γ chain; Common gamma chain; gamma c; SCIDX1
      Human,Dog (Tested in Development)
      Rat IgG2b, κ
      Human γc Transfected Cell Line
      Flow cytometry (Qualified)
      0.2 mg/ml
      VI C-89
      3561
      Aqueous buffered solution containing ≤0.09% sodium azide.
      RUO


      Preparation And Storage

      The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to the dye under optimum conditions that minimize unconjugated dye and antibody. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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      Recommended Assay Procedures

      BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application..

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      Product Notices

      1. The production process underwent stringent testing and validation to assure that it generates a high-quality conjugate with consistent performance and specific binding activity. However, verification testing has not been performed on all conjugate lots.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
      3. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      4. An isotype control should be used at the same concentration as the antibody of interest.
      5. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
      6. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
      7. Human donor specific background has been observed in relation to the presence of anti-polyethylene glycol (PEG) antibodies, developed as a result of certain vaccines containing PEG, including some COVID-19 vaccines. We recommend use of BD Horizon Brilliant™ Stain Buffer in your experiments to help mitigate potential background. For more information visit https://www.bdbiosciences.com/en-us/support/product-notices.
      8. Species cross-reactivity detected in product development may not have been confirmed on every format and/or application.
      9. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
      10. This product is provided under an Agreement between BIOTIUM and BD Biosciences. This product, and only in the amount purchased by buyer, may be used solely for buyer’s own internal research, in a manner consistent with the accompanying product literature. No other right to use, sell or otherwise transfer (a) this product, or (b) its components is hereby granted expressly, by implication or by estoppel. This product is for research use only. Diagnostic uses require a separate license from Biotium, Inc. For information on purchasing a license to this product including for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
      11. Alexa Fluor™ is a trademark of Life Technologies Corporation.
      12. For U.S. patents that may apply, see bd.com/patents.
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      752291 Rev. 2
      Antibody Details
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      TUGh4

      The TUGh4 monoclonal antibody specifically binds to CD132. CD132 is a 65-70 kDa type 1 transmembrane glycoprotein that is encoded by the IL2RG (interleukin 2 receptor, gamma) gene. CD132 is also known as the common γ subunit (γc) and is shared by the IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21 receptor complexes. CD132 is broadly expressed by most peripheral T and B lymphocytes, NK cells, monocytes, and granulocytes. The cytoplasmic domain of the γc chain plays an important role in cytokine-mediated signal transduction. Mutation of the IL2RG gene results in X-linked severe combined immunodeficiency (XSCID). The TUGh4 antibody recognizes a different epitope from that recognized by the CD132-specific clone, AG184.

      752291 Rev. 2
      Format Details
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      R718
      The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. Driven by BD innovation, R718 is designed to be excited by the red laser (627–640-nm) and detected using an optical filter centered near 720-nm (e.g., a 720/40-nm bandpass filter). R718 is a brighter alternative to Alexa Fluor™ 700. R718 is also a bright small molecule alternative to APC-R700 with lower spread into the APC detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
      altImg
      R718
      Red 627-640 nm
      695 nm
      718 nm
      752291 Rev.2
      Citations & References
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      View product citations for antibody "752291" on CiteAb

      Development References (4)

      1. Ishii N, Kondo M, Takeshita T, and Sugamura K. mAb specific for the γ chain of the IL-2 receptor. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:1867-1868.
      2. Ishii N, Takeshita T, Kimura Y, et al. Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood. Int Immunol. 1994; 6(8):1273-1277. (Immunogen: Flow cytometry, Immunoprecipitation, Radioimmunoassay). View Reference
      3. Matsuoka M, Takeshita T, Ishii N, Nakamura M, Ohkubo T, Sugamura K. Kinetic study of interleukin-2 binding on the reconstituted interleukin-2 receptor complexes including the human gamma chain. Eur J Immunol. 1993; 23(10):2472-2476. (Biology). View Reference
      4. Tanaka N, Sugamura K. CD132 (interleukin 2 γ chain (common γ) Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:861-864.
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      752291 Rev. 2

       

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      Please refer to Support Documents for Quality Certificates

       

      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

       

      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.