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      Purified NA/LE Hamster Anti-Rat CD29
      Product Details
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      BD Pharmingen™
      Itgb1; Integrin β1 chain; Integrin beta-1; VLA-4 subunit beta
      Rat (QC Testing), Mouse (Tested in Development)
      Armenian Hamster IgM, κ
      Rat glomerular epithelial cells
      Flow cytometry (Routinely Tested), Blocking, Immunoprecipitation (Reported)
      1.0 mg/ml
      AB_395636
      No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.
      RUO


      Preparation And Storage

      Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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      Product Notices

      1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
      2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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      555002 Rev. 10
      Antibody Details
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      Ha2/5

      The Ha2/5 monoclonal antibody specifically binds to the 130 kDa integrin β1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin α chains. With α1 through α6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with αV (CD51), it forms αVβ1 integrin. As a result, CD29 has a broad tissue distribution, including lymphocytes, endothelia, smooth muscle, and epithelia. The Ha2/5 hamster anti-rat CD29 monoclonal antibody cross-reacts with mouse thymocytes, splenocytes, and peripheral lymph node leukocytes. The Ha2/5 antibody blocks in vitro adhesion of CD29-expressing cells to collagen.

      555002 Rev. 10
      Format Details
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      NA/LE
      NA/LE refers to the culture and purification methods and buffer used to produce purified antibodies with no azide and low endotoxin: Aqueous buffered solution containing no preservative, 0.2µm sterile filtered. Endotoxin level is ≤0.01 EU/µg (≤0.001 ng/µg) of protein as determined by the LAL assay.NA/LE are perfectly suited to be used in culture or in vivo (for nonhuman studies) for functional assays — blocking, neutralizing, activation or depletion — where the presence of azide may damage cells or exogenous endotoxin may signal or activate cells.
      NA/LE
      555002 Rev.10
      Citations & References
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      View product citations for antibody "555002" on CiteAb

      Development References (2)

      1. Mendrick DL, Kelly DM. Temporal expression of VLA-2 and modulation of its ligand specificity by rat glomerular epithelial cells in vitro. Lab Invest. 1993; 69(6):690-702. (Immunogen: Blocking, Immunoprecipitation). View Reference
      2. Springer TA. Adhesion receptors of the immune system. Nature. 1990; 346(6283):425-434. (Biology). View Reference
      555002 Rev. 10

      Please refer to Support Documents for Quality Certificates


      Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


      Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

      For Research Use Only. Not for use in diagnostic or therapeutic procedures.